Chandran Sunil, Shapland Elaine
Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA, 94608, USA.
Methods Mol Biol. 2017;1472:187-92. doi: 10.1007/978-1-4939-6343-0_14.
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.
在大多数合成生物学实验室中，将多个DNA片段组装成更大的DNA构建体是一项必要操作。在此，我们描述了一种利用酵母同源重组（YHR）高效、高通量组装DNA的方法。YHR方法利用重叠的DNA片段，酿酒酵母通过设计的重叠区域之间的同源重组将这些片段组装在一起。使用这种方法，我们已成功在单个反应中组装多达12个DNA片段。
