Zelli R, Orlandi R, Verstegen J, Troisi A, Elad Ngonput A, Menchetti L, Cardinali L, Polisca A
Department Veterinary Medicine, Universita degli Studi di Perugia, Perugia, Italy.
Tyrus Clinica Veterinaria, Terni, Italy.
Andrology. 2017 Jan;5(1):160-168. doi: 10.1111/andr.12286. Epub 2016 Sep 27.
Prostasome-like vesicles (PV) are components of the canine prostatic fluid during ejaculation and despite some enzymatic activities have been identified recently, their functions in the reproductive events in this species are still poorly understood. In this study, we evaluated at neutral or slightly alkaline pH, the effects of different concentrations of purified PV on sperm cell motility characteristics and the impact on the short- and long-term preservation of preserved semen maintained at room temperature. Two different experiments were performed. In the first experiment, purified PV were added at increasing concentration (1.25, 2.5, 5, 10, and 20 μL equivalent to 1, 2, 4, 8, and 16 μg of protein, respectively) to aliquots of 100 μL of preserved semen maintained at 22 °C at the following time points: 0, 30, 60, 120, 240, and 480 min. Computer-assisted sperm motility characteristics and pH were assessed three times at each time points, for each sample and for every concentrations. In the second experiment, the purified PV were added with the same methods as described above but only at time 0. Sperm motility characteristics and pH were assessed over the time. This study showed how the addition of purified PV to preserved semen affects negatively (p < 0.05) at neutral and alkaline pH, both total and progressive motility in a concentration depending manner. Furthermore, prostasome addition was demonstrated to change the quality of sperm movement which may represent a mechanism facilitating sperm cells attachment to the uterine epithelium and facilitating energy preservation before fertilization.
前列腺小体样囊泡（PV）是犬射精时前列腺液的组成部分，尽管最近已鉴定出一些酶活性，但其在该物种生殖过程中的功能仍知之甚少。在本研究中，我们在中性或略碱性pH条件下，评估了不同浓度的纯化PV对精子细胞运动特征的影响，以及对室温保存精液的短期和长期保存的影响。进行了两个不同的实验。在第一个实验中，将纯化的PV以递增浓度（分别为1.25、2.5、5、10和20 μL，相当于1、2、4、8和16 μg蛋白质）添加到100 μL保存在22°C的精液等分试样中，时间点如下：0、30、60、120、240和480分钟。在每个时间点，对每个样品和每个浓度的精子运动特征和pH进行三次计算机辅助评估。在第二个实验中，以与上述相同的方法添加纯化的PV，但仅在时间0添加。随时间评估精子运动特征和pH。本研究表明，在中性和碱性pH条件下，向保存的精液中添加纯化的PV会以浓度依赖的方式对总运动性和渐进性运动性产生负面影响（p < 0.05）。此外，已证明添加前列腺小体可改变精子运动的质量，这可能代表一种促进精子细胞附着于子宫上皮并在受精前促进能量保存的机制。
