Dong Xiangrong, Wang Yanping, Yang Fengyuan, Zhao Shanshan, Tian Bing, Li Tao
Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Biotechnol Lett. 2017 Jan;39(1):65-70. doi: 10.1007/s10529-016-2220-2. Epub 2016 Sep 27.
Lycopene biosynthetic genes from Deinococcus radiodurans were co-expressed in Lactococcus lactis to produce lycopene and improve its tolerance to stress.
Lycopene-related genes from D. radiodurans, DR1395 (crtE), DR0862 (crtB), and DR0861 (crtI), were fused in line with S hine-Dalgarno (SD) sequences and co-expressed in L. lactis. The recombinant strain produced 0.36 mg lycopene g dry cell wt after 48 h fermentation. The survival rate to UV irradiation of the recombinant strain was higher than that of the non-transformed strain.
The L. lactis with co-expressed genes responsible for lycopene biosynthesis from D. radiodurans produced lycopene and exhibited increased resistance to UV stress, suggesting that the recombinant strain has important application potential in food industry.
将来自耐辐射球菌的番茄红素生物合成基因在乳酸乳球菌中进行共表达，以生产番茄红素并提高其对胁迫的耐受性。
来自耐辐射球菌的与番茄红素相关的基因，DR1395（crtE）、DR0862（crtB）和DR0861（crtI），与Shine-Dalgarno（SD）序列融合并在乳酸乳球菌中共表达。重组菌株在48小时发酵后产生了0.36毫克番茄红素/克干细胞重。重组菌株对紫外线照射的存活率高于未转化菌株。
共表达来自耐辐射球菌的负责番茄红素生物合成基因的乳酸乳球菌产生了番茄红素，并表现出对紫外线胁迫的抗性增加，表明该重组菌株在食品工业中具有重要的应用潜力。
