碳水化合物残基在人免疫球蛋白G和治疗性单克隆抗体中的功能作用

Functional Role of Carbohydrate Residues in Human Immunoglobulin G and Therapeutic Monoclonal Antibodies.

作者信息

Dorokhov Y L, Sheshukova E V, Kosobokova E N, Shindyapina A V, Kosorukov V S, Komarova T V

机构信息

Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia.

出版信息

Biochemistry (Mosc). 2016 Aug;81(8):835-57. doi: 10.1134/S0006297916080058.

DOI:10.1134/S0006297916080058

PMID:27677552

Abstract

Therapeutic monoclonal antibodies (TMA) provide an important means for treating diseases that were previously considered untreatable. Currently more than 40 full-size TMAs created primarily based on immunoglobulin G1 are widely used for treating various illnesses. Glycosylation of TMA is among other numerous factors that affect their biological activity, effector functions, immunogenicity, and half-life in the patient's serum. The importance of carbohydrate residues for activity of human serum immunoglobulin and TMA produced in animal cells is considered in this review, with emphasis given to N-glycosylation of the Fc fragment of the antibody.

摘要

治疗性单克隆抗体（TMA）为治疗以前被认为无法治疗的疾病提供了重要手段。目前，主要基于免疫球蛋白G1产生的40多种全尺寸TMA被广泛用于治疗各种疾病。TMA的糖基化是影响其生物学活性、效应功能、免疫原性以及在患者血清中半衰期的众多因素之一。本文综述了碳水化合物残基对人血清免疫球蛋白和动物细胞产生的TMA活性的重要性，重点讨论了抗体Fc片段的N-糖基化。