Li Yanxi, Chen Long, Du Yehong, Huang Daochao, Han Huili, Dong Zhifang
Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Children's Hospital of Chongqing Medical UniversityChongqing, China; Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical UniversityChongqing, China; The Chongqing Hospital of Traditional Chinese MedicineChongqing, China.
Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Children's Hospital of Chongqing Medical UniversityChongqing, China; Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical UniversityChongqing, China.
Front Pharmacol. 2016 Sep 13;7:318. doi: 10.3389/fphar.2016.00318. eCollection 2016.
Atopic dermatitis (AD) is a common chronic inflammatory skin disorder, and patients with AD suffer from severe psychological stress, which markedly increases the prevalence rate of depression and anxiety disorders in later life. Fluoxetine, a selective serotonin reuptake inhibitor, has recently been reported to exert anti-inflammatory and immunosuppressive effects. However, it is unclear whether fluoxetine is effective in the treatment of AD through reducing psychological stress and inflammatory reaction. Here, we reported that a BALB/c mouse model of AD was induced by application of 2,4-dinitrochlorobenzene (DNCB) onto hairless dorsal skin. Chronic fluoxetine treatment (10 mg/kg per day, i.p.) significantly attenuated AD-like symptoms, as reflected by a dramatic decrease in scratching bouts, as well as a decrease in anxiety- and depressive-like behaviors. Furthermore, these behavioral changes were accompanied by a significant decrease in epidermal thickness, the number of mast cells in skin tissue, mRNA levels of interleukin-4 (IL-4) and IL-13 in the spleen, as well as serum immunoglobulin E (IgE) in the DNCB-treated mice by treatment with fluoxetine. Taken together, these results indicate that fluoxetine may suppress psychological stress and inflammatory response during AD development, and subsequently ameliorate AD symptoms, suggesting that fluoxetine may be a potential therapeutic agent against AD in clinic.
特应性皮炎（AD）是一种常见的慢性炎症性皮肤病，AD患者承受着严重的心理压力，这显著增加了其在晚年患抑郁症和焦虑症的患病率。氟西汀是一种选择性5-羟色胺再摄取抑制剂，最近有报道称它具有抗炎和免疫抑制作用。然而，尚不清楚氟西汀是否通过减轻心理压力和炎症反应来有效治疗AD。在此，我们报道通过将2,4-二硝基氯苯（DNCB）涂抹于无毛背部皮肤诱导建立AD的BALB/c小鼠模型。慢性氟西汀治疗（每天10mg/kg，腹腔注射）显著减轻了AD样症状，表现为搔抓发作次数显著减少，以及焦虑样和抑郁样行为减少。此外，这些行为变化伴随着表皮厚度、皮肤组织中肥大细胞数量、脾脏中白细胞介素-4（IL-4）和IL-13的mRNA水平以及经DNCB处理的小鼠血清免疫球蛋白E（IgE）的显著降低，这是氟西汀治疗的结果。综上所述，这些结果表明氟西汀可能在AD发展过程中抑制心理压力和炎症反应，随后改善AD症状，提示氟西汀可能是临床上治疗AD的一种潜在治疗药物。
