van Hooij Anouk, Tjon Kon Fat Elisa M, Richardus Renate, van den Eeden Susan J F, Wilson Louis, de Dood Claudia J, Faber Roel, Alam Korshed, Richardus Jan Hendrik, Corstjens Paul L A M, Geluk Annemieke
Dept. of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Dept. Molecular Cell Biology, Leiden University Medical Center, The Netherlands.
Sci Rep. 2016 Sep 29;6:34260. doi: 10.1038/srep34260.
Leprosy is a debilitating, infectious disease caused by Mycobacterium leprae. Despite the availability of multidrug therapy, transmission is unremitting. Thus, early identification of M. leprae infection is essential to reduce transmission. The immune response to M. leprae is determined by host genetics, resulting in paucibacillary (PB) and multibacillary (MB) leprosy associated with dominant cellular or humoral immunity, respectively. This spectral pathology of leprosy compels detection of immunity to M. leprae to be based on multiple, diverse biomarkers. In this study we have applied quantitative user friendly lateral flow assays (LFAs) for four immune markers (anti-PGL-I antibodies, IL-10, CCL4 and IP-10) for whole blood samples from a longitudinal BCG vaccination field-trial in Bangladesh. Different biomarker profiles, in contrast to single markers, distinguished M. leprae infected from non-infected test groups, patients from household contacts (HHC) and endemic controls (EC), or MB from PB patients. The test protocol presented in this study merging detection of innate, adaptive cellular as well as humoral immunity, thus provides a convenient tool to measure specific biomarker profiles for M. leprae infection and leprosy utilizing a field-friendly technology.
麻风病是一种由麻风分枝杆菌引起的使人衰弱的传染病。尽管有多种药物疗法，但传播仍在持续。因此，早期识别麻风分枝杆菌感染对于减少传播至关重要。对麻风分枝杆菌的免疫反应由宿主基因决定，分别导致与占主导地位的细胞免疫或体液免疫相关的少菌型（PB）和多菌型（MB）麻风病。麻风病这种光谱病理学特征使得对麻风分枝杆菌免疫力的检测必须基于多种不同的生物标志物。在本研究中，我们对来自孟加拉国一项卡介苗接种纵向现场试验的全血样本，应用了针对四种免疫标志物（抗酚糖脂-I抗体、白细胞介素-10、趋化因子配体4和干扰素诱导蛋白10）的定量且用户友好的侧向流动分析（LFA）。与单一标志物相比，不同的生物标志物谱能够区分麻风分枝杆菌感染组与未感染组、患者与家庭接触者（HHC）及地方性对照（EC），或者区分多菌型患者与少菌型患者。本研究中提出的检测方案融合了对固有免疫、适应性细胞免疫以及体液免疫的检测，因此提供了一种便捷工具，可利用一种便于现场操作的技术来测量针对麻风分枝杆菌感染和麻风病的特定生物标志物谱。
