Grubb M F, Callery P S
Department of Medicinal Chemistry/Pharmacognosy, University of Maryland, Baltimore 21201.
J Chromatogr. 1989 May 19;469:191-6. doi: 10.1016/s0021-9673(01)96454-4.
Six amino acids containing either an N-methyl or a cyclic secondary amine were converted to volatile derivatives by reaction with dimethylformamide dimethyl acetal. The amine functionalities were formylated by way of an amide acetal intermediate while the carboxylic acid groups were esterified directly. The resulting N-formyl esters were stable to solvent extraction and exhibited gas chromatography-mass spectrometry properties suitable for assay development.
通过与二甲基甲酰胺二甲基缩醛反应，将六种含有N-甲基或环状仲胺的氨基酸转化为挥发性衍生物。胺官能团通过酰胺缩醛中间体进行甲酰化，而羧酸基团则直接酯化。所得的N-甲酰酯对溶剂萃取稳定，并具有适合分析方法开发的气相色谱-质谱特性。
