Poll D J, Harding D R
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
J Chromatogr. 1989 May 19;469:231-9. doi: 10.1016/s0021-9673(01)96458-1.
In reversed-phase chromatography of peptides, formic acid has been shown to successfully replace the stronger traditional trifluoroacetic and phosphoric acids. Detection of non-aromatic peptide at lower wavelength is not impaired and being volatile the acid is easily removed, enabling further studies of the peptides. Also in ion-exchange chromatography (the first step of a two-dimensional approach) formic acid works well with a sulphonic acid ion-exchange resin.
