Reihmane Dace, Gram Martin, Vigelsø Andreas, Wulff Helge Jørn, Dela Flemming
a Institute of Clinical and Experimental Medicine , University of Latvia , Riga , Latvia.
b Xlab, Center for Healthy Aging, Department of Biomedical Sciences, Faculty of Health Sciences , University of Copenhagen , Copenhagen , Denmark.
Eur J Sport Sci. 2016 Nov;16(8):1039-46. doi: 10.1080/17461391.2015.1111939. Epub 2015 Nov 19.
Physical inactivity is a major contributor to low-grade systemic inflammation. Most of the studies characterizing interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) release from exercising legs have been done in young, healthy men, but studies on inactivity in older people are lacking. The impact of 14 days of one-leg immobilization (IM) on IL-6 and TNF-α release during exercise in comparison to the contralateral control (CON) leg was investigated. Fifteen healthy men (age 68.1 ± 1.1 year (mean ± SEM); BMI 27.0 ± 0.4 kg·m(2); VO2max 33.3 ± 1.6 ml·kg(‒1)·min(‒1)) performed 45 min of two-leg dynamic knee extensor exercise at 19.5 ± 0.9 W. Arterial and femoral venous blood samples from the CON and the IM legs were collected every 15 min during exercise, and thigh blood flow was measured with ultrasound Doppler. Arterial plasma IL-6 concentration increased with exercise (rest vs. 45 min, main effect p < .05). IL-6 release increased with exercise (rest vs. 30 min, main effect p < .05). Furthermore, IL-6 release was borderline (main effect, p = .085, effect size 0.28) higher in the IM leg compared to the CON leg (288 (95% CI: 213-373) vs. 220 (95% CI: 152-299) pg·min(‒1), respectively). There was no release of TNF-α in either leg and arterial concentrations remained unchanged during exercise (p > .05). In conclusion, exercise induces more pronounced IL-6 secretion in healthy older men. Two weeks of unilateral immobilization on the other hand had only a minor influence on IL-6 release. Neither immobilization nor exercise had an effect on TNF-α release across the working legs in older men.
缺乏身体活动是导致低度全身炎症的主要因素。大多数关于运动腿部释放白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的研究都是在年轻健康男性中进行的，但关于老年人缺乏身体活动的研究却很匮乏。本研究调查了与对侧对照（CON）腿相比，单腿固定（IM）14天对运动期间IL-6和TNF-α释放的影响。15名健康男性（年龄68.1±1.1岁（均值±标准误）；体重指数27.0±0.4kg·m²；最大摄氧量33.3±1.6ml·kg⁻¹·min⁻¹）以19.5±0.9W的功率进行了45分钟的双腿动态伸膝运动。运动期间每15分钟采集CON腿和IM腿的动脉血和股静脉血样本，并用超声多普勒测量大腿血流量。动脉血浆IL-6浓度随运动增加（静息状态与45分钟时相比，主效应p<0.05）。IL-6释放量随运动增加（静息状态与30分钟时相比，主效应p<0.05）。此外，IM腿的IL-6释放量比CON腿略高（主效应，p = 0.085，效应大小为（288（95%置信区间：213 - 373）与220（95%置信区间：152 - 299）pg·min⁻¹）。两条腿均未释放TNF-α，运动期间动脉浓度保持不变（p>0.05）。总之，运动在健康老年男性中诱导出更明显的IL-6分泌。另一方面，两周的单侧固定对IL-6释放只有轻微影响。在老年男性中，固定和运动对工作腿部的TNF-α释放均无影响。
