Lou Dan, Wang Qiaochu, Huang Min, Zhou Zhijun
a School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health , Fudan University , Shanghai , China.
b Shanghai Municipal Center for Disease Control and Prevention , Shanghai , China.
Toxicol Mech Methods. 2016 Nov;26(9):667-673. doi: 10.1080/15376516.2016.1223241. Epub 2016 Nov 6.
Epidemiological studies have revealed that environmentally relevant low levels of paraquat (PQ) exposure is listed on the etiology of neurological disorders such as Parkinson's disease (PD). The behavioral effects of PQ are of current interest, especially when exposure occurs in the period of early stage of life. To characterize whether and how age affects neurobehavioral performances of mice after PQ exposure, 21 days postnatal (PN21) and adult male C57BL/6 mice were daily administrated by oral gavage with 0 mg/kg (saline, control), 5 mg/kg or 10 mg/kg of PQ for 28 consecutive days. Survival rate and body weight were analyzed. Subsequently, mice were subjected to Morris water maze tests (MWM). The results showed that mice exposed to PQ had significantly longer latencies than those in the control group, with a dose-dependent manner. Furthermore, PN21 mice tended to have longer latencies than adult mice in the same dose group. Our data suggested that PQ exposure induced significant learning and memory impairment and more severely in PN21 mice when compared with adult mice.
流行病学研究表明，与环境相关的低水平百草枯（PQ）暴露被列为帕金森病（PD）等神经疾病的病因之一。PQ的行为效应是当前研究的热点，尤其是在生命早期阶段发生暴露时。为了确定年龄是否以及如何影响PQ暴露后小鼠的神经行为表现，对出生后21天（PN21）和成年雄性C57BL/6小鼠连续28天每天经口灌胃给予0毫克/千克（生理盐水，对照组）、5毫克/千克或10毫克/千克的PQ。分析存活率和体重。随后，对小鼠进行莫里斯水迷宫试验（MWM）。结果显示，暴露于PQ的小鼠潜伏期明显长于对照组，且呈剂量依赖性。此外，在相同剂量组中，PN21小鼠的潜伏期往往比成年小鼠更长。我们的数据表明，与成年小鼠相比，PQ暴露会导致显著的学习和记忆障碍，且在PN21小鼠中更为严重。
