Nie Ying, Wang Da-Qing, Zhao Guang, Yu Song, Wang Hong-Yan
College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China; Heilongjiang State Farms Institute of Economy, Harbin 150090, China.
Heilongjiang State Farms Institute of Economy, Harbin 150090, China.
Biomed Res Int. 2016;2016:4904087. doi: 10.1155/2016/4904087. Epub 2016 Sep 4.
The development of transgenic soybean has produced numerous economic benefits; however the potential impact of root exudates upon soil ecological systems and rhizospheric soil microbial diversity has also received intensive attention. In the present study, the influence of saline-alkali tolerant transgenic soybean of betaine aldehyde dehydrogenase on bacterial community structure and soil phosphatase during growth stages was investigated. The results showed that, compared with nontransgenic soybean as a control, the rhizospheric soil pH of transgenic soybean significantly decreased at the seedling stage. Compared to HN35, organic P content was 13.5% and 25.4% greater at the pod-filling stage and maturity, respectively. The acid phosphatase activity of SRTS was significantly better than HN35 by 12.74% at seedling, 14.03% at flowering, and 59.29% at podding, while alkaline phosphatase achieved maximum activity in the flowering stage and was markedly lower than HN35 by 13.25% at pod-filling. The 454 pyrosequencing technique was employed to investigate bacterial diversity, with a total of 25,499 operational taxonomic units (OTUs) obtained from the 10 samples. Notably, the effect of SRTS on microbial richness and diversity of rhizospheric soil was marked at the stage of podding and pod-filling. Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla among all samples. Compared with HN35, the relative abundance of Proteobacteria was lower by 2.01%, 2.06%, and 5.28% at the stage of seedling, at pod-bearing, and at maturity. In genus level, the relative abundance of Gp6, sp., and GP4 was significantly inhibited by SRTS at the stage of pod-bearing and pod-filling.
转基因大豆的发展带来了诸多经济效益；然而，根系分泌物对土壤生态系统和根际土壤微生物多样性的潜在影响也受到了广泛关注。在本研究中，调查了耐盐碱转基因甜菜碱醛脱氢酶大豆在生长阶段对细菌群落结构和土壤磷酸酶的影响。结果表明，与作为对照的非转基因大豆相比，转基因大豆根际土壤pH在苗期显著降低。与HN35相比，在鼓粒期和成熟期有机磷含量分别高出13.5%和25.4%。耐盐碱转基因大豆（SRTS）的酸性磷酸酶活性在苗期显著优于HN35，高出12.74%，在花期高出14.03%，在结荚期高出59.29%，而碱性磷酸酶在花期达到最大活性，在鼓粒期显著低于HN35，低13.25%。采用454焦磷酸测序技术研究细菌多样性，从10个样品中共获得25499个可操作分类单元（OTU）。值得注意的是，耐盐碱转基因大豆对根际土壤微生物丰富度和多样性的影响在结荚期和鼓粒期较为显著。变形菌门、酸杆菌门和放线菌门是所有样品中的优势菌门。与HN35相比，在苗期、结荚期和成熟期，变形菌门的相对丰度分别降低了2.01%、2.06%和5.28%。在属水平上，在结荚期和鼓粒期，耐盐碱转基因大豆对Gp6、sp.和GP4的相对丰度有显著抑制作用。
