Ding Ya, Song Zhiwen, Liu Jinbo
Department of Orthopedics, The Third Affiliated Hospital, Soochow University, Changzhou, Jiangsu, China; Department of Orthopedic Surgery, Fuyang People's Hospital, Anhui Medical University, No. 63 Luci Street, Fuyang City, Anhui 236004, China.
Department of Orthopedics, The Third Affiliated Hospital, Soochow University, Changzhou, Jiangsu, China.
Biomed Res Int. 2016;2016:9249401. doi: 10.1155/2016/9249401. Epub 2016 Sep 4.
Long noncoding RNAs (LncRNAs) play a crucial role in cell growth, development, and various diseases related to the central nervous system. However, LncRNA differential expression profiles in spinal cord injury are yet to be reported. In this study, we profiled the expression pattern of LncRNAs using a microarray method in a contusion spinal cord injury (SCI) mouse model. Compared with a spinal cord without injury, few changes in LncRNA expression levels were noted 1 day after injury. The differential changes in LncRNA expression peaked 1 week after SCI and subsequently declined until 3 weeks after injury. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the reliability of the microarray, demonstrating that the results were reliable. Gene ontology (GO) analysis indicated that differentially expressed mRNAs were involved in transport, cell adhesion, ion transport, and metabolic processes, among others. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the neuroactive ligand-receptor interaction, the PI3K-Akt signaling pathway, and focal adhesions were potentially implicated in SCI pathology. We constructed a dynamic LncRNA-mRNA network containing 264 LncRNAs and 949 mRNAs to elucidate the interactions between the LncRNAs and mRNAs. Overall, the results from this study indicate for the first time that LncRNAs are differentially expressed in a contusion SCI mouse model.
长链非编码RNA（LncRNAs）在细胞生长、发育以及与中枢神经系统相关的各种疾病中发挥着关键作用。然而，脊髓损伤中LncRNA的差异表达谱尚未见报道。在本研究中，我们采用微阵列方法在挫伤性脊髓损伤（SCI）小鼠模型中分析了LncRNAs的表达模式。与未损伤的脊髓相比，损伤后1天LncRNA表达水平几乎没有变化。LncRNA表达的差异变化在脊髓损伤后1周达到峰值，随后下降直至损伤后3周。采用定量实时聚合酶链反应（qRT-PCR）验证微阵列结果的可靠性，结果表明该结果可靠。基因本体（GO）分析表明，差异表达的mRNA参与了运输、细胞黏附、离子运输和代谢过程等。京都基因与基因组百科全书（KEGG）富集分析显示，神经活性配体-受体相互作用、PI3K-Akt信号通路和黏着斑可能与脊髓损伤病理相关。我们构建了一个包含264个LncRNAs和949个mRNAs的动态LncRNA-mRNA网络，以阐明LncRNAs与mRNAs之间的相互作用。总体而言，本研究结果首次表明LncRNAs在挫伤性脊髓损伤小鼠模型中存在差异表达。
