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过表达XIST的间充质干细胞诱导巨噬细胞M2极化并改善神经干细胞稳态微环境，减轻脊髓损伤。

Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury.

作者信息

Zhu Dan, Peng Tie, Zhang Zhenwang, Guo Shuang, Su Ying, Zhang Kangwei, Wang Jiawei, Liu Chao

机构信息

Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China.

Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China.

出版信息

J Tissue Eng. 2024 Jan 10;15:20417314231219280. doi: 10.1177/20417314231219280. eCollection 2024 Jan-Dec.

DOI:10.1177/20417314231219280
PMID:38223166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10785713/
Abstract

Spinal cord injury (SCI) is a significant cause of disability worldwide, with limited treatment options. This study investigated the potential of bone marrow-derived mesenchymal stem cells (BMSCs) modified with XIST lentiviral vector to modulate macrophage polarization and affect neural stem cell (NSC) microenvironment reconstruction following SCI. Bioinformatics analysis revealed that MID1 might be crucial for BMSCs' treatment of SCI. XIST overexpression enriched Zmynd8 to the promoter region of MID1 and inhibited MID1 transcription, which promoted macrophage M2 polarization. In vitro experiments showed that BMSCs-XIST promoted NSC proliferation, migration, differentiation, and axonal growth by inducing macrophage M2 polarization, suppressing inflammation, and accelerating the re-establishment of the homeostatic microenvironment of NSCs. In vivo, animal experiments confirmed that BMSCs-XIST significantly alleviated SCI by promoting NSC differentiation and axon formation in the injured area. The study demonstrated the potential of XIST-overexpressing BMSCs for treating SCI by regulating macrophage polarization and homeostasis of the NSC microenvironment. These findings provide new insights into the development of stem cell-based therapies for SCI.

摘要

脊髓损伤（SCI）是全球范围内导致残疾的一个重要原因，治疗选择有限。本研究调查了用XIST慢病毒载体修饰的骨髓间充质干细胞（BMSCs）调节巨噬细胞极化以及影响SCI后神经干细胞（NSC）微环境重建的潜力。生物信息学分析表明，MID1可能对BMSCs治疗SCI至关重要。XIST过表达使Zmynd8富集到MID1的启动子区域并抑制MID1转录，从而促进巨噬细胞M2极化。体外实验表明，BMSCs-XIST通过诱导巨噬细胞M2极化、抑制炎症以及加速NSCs稳态微环境的重新建立，促进了NSC的增殖、迁移、分化和轴突生长。在体内，动物实验证实BMSCs-XIST通过促进损伤区域的NSC分化和轴突形成，显著减轻了SCI。该研究证明了过表达XIST的BMSCs通过调节巨噬细胞极化和NSC微环境的稳态来治疗SCI的潜力。这些发现为基于干细胞的SCI治疗方法的开发提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/a0e3d9274405/10.1177_20417314231219280-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/51f8f2d39c22/10.1177_20417314231219280-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/428121a60a19/10.1177_20417314231219280-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/2e812d749e4c/10.1177_20417314231219280-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/0c6e3efa1234/10.1177_20417314231219280-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/b221913b4006/10.1177_20417314231219280-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/670012d4f6cb/10.1177_20417314231219280-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/6b1a8edd508d/10.1177_20417314231219280-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/a0e3d9274405/10.1177_20417314231219280-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/51f8f2d39c22/10.1177_20417314231219280-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/428121a60a19/10.1177_20417314231219280-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/2e812d749e4c/10.1177_20417314231219280-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/0c6e3efa1234/10.1177_20417314231219280-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/b221913b4006/10.1177_20417314231219280-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/670012d4f6cb/10.1177_20417314231219280-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/6b1a8edd508d/10.1177_20417314231219280-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11b5/10785713/a0e3d9274405/10.1177_20417314231219280-fig8.jpg

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3
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4
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5
The role of MUC16 in tumor biology and tumor immunology in ovarian cancer.MUC16在卵巢癌肿瘤生物学和肿瘤免疫学中的作用。
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6
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