Gómez Erika Olivia, Chirino Yolanda Irasema, Delgado-Buenrostro Norma Laura, López-Saavedra Alejandro, Meraz-Cruz Noemí, López-Marure Rebeca
a Universidad Autónoma de la Ciudad de México, Colegio de Ciencias y Humanidades , Plantel San Lorenzo Tezonco , México.
b Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM , México.
Mol Membr Biol. 2016 Mar;33(1-2):29-37. doi: 10.1080/09687688.2016.1229057. Epub 2016 Sep 30.
Metastases, responsible for most of the solid tumor associated deaths, require angiogenesis and changes in endothelial cells. In this work, the effect of the secretomes of three breast tumor cell lines (MCF-7, MDA-MB-231 and ZR-75-30) on human umbilical vein endothelial cells (HUVEC) morphology was investigated. HUVEC treated with secretomes from breast cells were analyzed by confocal and time-lapse microscopy. Secretomes from ZR-75-30 and MDA-MB-231 cells modify the morphology and adhesion of HUVEC. These changes may provoke the loss of endothelial monolayer integrity. In consequence, tumor cells could have an increased access to circulation, which would then enhance metastasis.
转移是导致大多数实体瘤相关死亡的原因，它需要血管生成以及内皮细胞的变化。在这项研究中，研究了三种乳腺癌细胞系（MCF-7、MDA-MB-231和ZR-75-30）的分泌产物对人脐静脉内皮细胞（HUVEC）形态的影响。用乳腺细胞分泌产物处理的HUVEC通过共聚焦显微镜和延时显微镜进行分析。ZR-75-30和MDA-MB-231细胞的分泌产物改变了HUVEC的形态和黏附。这些变化可能导致内皮单层完整性的丧失。因此，肿瘤细胞可能更容易进入循环系统，进而增强转移。
