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足月儿对白细胞介素-8和细菌N-甲酰肽的中性粒细胞趋化反应差异

Differential neutrophil chemotactic response towards IL-8 and bacterial N-formyl peptides in term newborn infants.

作者信息

Stålhammar Maria E, Douhan Håkansson Lena, Jonzon Anders, Sindelar Richard

机构信息

a Department of Women's and Children's Health , Uppsala University , Uppsala , Sweden.

b Department of Medical Sciences , Uppsala University , Uppsala , Sweden.

出版信息

Ups J Med Sci. 2017 Mar;122(1):35-42. doi: 10.1080/03009734.2016.1228721. Epub 2016 Oct 3.

DOI:10.1080/03009734.2016.1228721
PMID:27690722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5361430/
Abstract

BACKGROUND

A prerequisite for an effective innate immunity is the migrative ability of neutrophils to respond to inflammatory and infectious agents such as the intermediate interleukin (IL)-8 and the end-target formyl-methionyl-leucyl-phenylalanine (fMLP) chemoattractants. The aim was to study the chemotactic capacity of neutrophils from newborn infants and adults in response to IL-8 and the bacterial peptide fMLP.

METHODS

In the under-agarose cell migration assay, isolated leukocytes from healthy adults and from cord blood of healthy term newborn infants were studied with dose responses towards IL-8 and fMLP. The same number of leukocytes (1 × 10 cells), with the same distribution of neutrophils and monocytes, were analyzed in neonates and adults. Chemotaxis was distinguished from randomly migrating neutrophils, and the neutrophil pattern of migration, i.e. the migration distance and the number of migrating neutrophils per distance, was evaluated.

RESULTS

In comparison to adults, fewer neutrophils from newborn infants migrated towards IL-8 and for a shorter distance (P < .01, respectively). The number of neutrophils migrating to different gradients of fMLP, the distance they migrated, and the correlation between the number and the distance were the same for neonates and adults. Random migration did not differ in any instance.

CONCLUSION

Chemotaxis of neutrophils from newborn infants was as co-ordinated as neutrophils from adults in response to fMLP, whereas the response to IL-8 was reduced. The differential response of neutrophils from neonates to intermediate and end-target chemoattractants could indicate a reduced infectious response.

摘要

背景

有效的先天免疫的一个先决条件是中性粒细胞的迁移能力，以响应炎症和感染因子，如中间型白细胞介素（IL）-8和终末靶标形式的甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）趋化因子。目的是研究新生儿和成人中性粒细胞对IL-8和细菌肽fMLP的趋化能力。

方法

在琼脂糖下细胞迁移试验中，研究了从健康成人和健康足月儿脐带血中分离的白细胞对IL-8和fMLP的剂量反应。在新生儿和成人中分析相同数量的白细胞（1×10个细胞），中性粒细胞和单核细胞的分布相同。趋化作用与随机迁移的中性粒细胞区分开来，并评估中性粒细胞的迁移模式，即迁移距离和每距离迁移的中性粒细胞数量。

结果

与成人相比，新生儿的中性粒细胞向IL-8迁移的数量更少，距离更短（分别为P＜0.01）。新生儿和成人迁移到不同梯度fMLP的中性粒细胞数量、迁移距离以及数量与距离之间的相关性相同。在任何情况下，随机迁移均无差异。

结论

新生儿中性粒细胞对fMLP的趋化作用与成人中性粒细胞一样协调，而对IL-8的反应则降低。新生儿中性粒细胞对中间型和终末靶标趋化因子的不同反应可能表明感染反应降低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/f93a52b16ca3/iups-122-35.F05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/af095c6c2291/iups-122-35.F01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/2356f80b7f02/iups-122-35.F02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/7892e91c2e36/iups-122-35.F03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/3748b02631d0/iups-122-35.F04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/f93a52b16ca3/iups-122-35.F05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/af095c6c2291/iups-122-35.F01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/2356f80b7f02/iups-122-35.F02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/7892e91c2e36/iups-122-35.F03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/3748b02631d0/iups-122-35.F04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fbc/5361430/f93a52b16ca3/iups-122-35.F05.jpg

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