Chung H M, Neff A W, Malacinski G M
Department of Biology, College of Education, Seoul National University, Korea.
J Exp Zool. 1989 Sep;251(3):290-9. doi: 10.1002/jez.1402510305.
The death of cranial myotomes during Xenopus laevis embryogenesis is employed as a model system to study programmed cell death. The first primary myotomes to differentiate and functionally develop are in the occipital region of the embryonic head. Between stages 27 (tailbud) and 48 (feeding tadpole), they degenerate and disappear in a craniocaudal sequence. Descriptive and experimental studies were undertaken to establish whether this apparent cell (myotome) death program is autonomous or whether it depends on interactions with surrounding tissues (e.g., otic vesicle). Removal of the adjacent otic vesicle did not affect cranial myotome death. Likewise, grafting the otic vesicle to a novel location along the somite file did not induce local myotome degeneration (death). Cranial myotome primordia grafted into the trunk region degenerated on schedule. Trunk myotome primordia grafted to the cranial myotome location did not degenerate. It is therefore concluded that the cranial myotome death program has become autonomous by the time the cranial myotomes reach the developmental stage of segmentation.
非洲爪蟾胚胎发育过程中颅部肌节的死亡被用作研究程序性细胞死亡的模型系统。最早分化并开始功能发育的初级肌节位于胚胎头部的枕部区域。在第27期（尾芽期）到第48期（摄食蝌蚪期）之间，它们按头尾顺序退化并消失。开展了描述性和实验性研究，以确定这种明显的细胞（肌节）死亡程序是自主的，还是依赖于与周围组织（如耳泡）的相互作用。移除相邻的耳泡并不影响颅部肌节的死亡。同样，将耳泡移植到沿体节列的新位置也不会诱导局部肌节退化（死亡）。移植到躯干区域的颅部肌节原基按时退化。移植到颅部肌节位置的躯干肌节原基不会退化。因此得出结论，当颅部肌节达到分节的发育阶段时，颅部肌节死亡程序已变得自主。
