Park Meerim, Park Chan-Jeoung, Cho Young Wook, Jang Seongsoo, Lee Jung-Hee, Lee Je-Hwan, Lee Kyoo-Hyung, Lee Young Ho
Department of Pediatrics, College of Medicine Chungbuk National University, Cheongju, Korea.
Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Exp Hematol. 2017 Jan;45:56-63. doi: 10.1016/j.exphem.2016.09.009. Epub 2016 Sep 28.
Hematopoiesis involves complex interactions between hematopoietic cells and the bone marrow (BM) microenvironment. The specific causes and mechanisms underlying dysregulated hematopoiesis are unknown. Here, BM biopsy specimens from patients with aplastic anemia (AA) and chronic myeloid leukemia (CML) and normal marrow were analyzed by semiquantitative immunohistochemistry to determine changes in the hematopoietic stem cell (HSC) compartment and BM microenvironment. HSC levels were lowest in AA and highest in CML. T and B lymphocytes were decreased in AA (p < 0.01) and CML (p < 0.01). Natural killer cells were observed in AA, but were absent in CML and healthy controls (p < 0.01). Macrophages and mast cells were absent in CML. There were significant differences between AA and CML stromal cell components. No nestin cells were observed in CML and the mean number of stromal cell-derived factor-1-positive cells was lowest in CML. Osteopontin cells were higher in AA than in CML (p < 0.01); osteonectin cells were higher in CML than in AA (p < 0.01). There was no significant difference in the expression of osteocalcin between AA and CML. The number of endothelial cells was highest in CML and lowest in AA (p < 0.01). Our findings suggest that changes in BM microenvironment components might be related to defective hematopoiesis leading to AA and/or CML.
造血作用涉及造血细胞与骨髓微环境之间复杂的相互作用。造血功能失调的具体原因和机制尚不清楚。在此，通过半定量免疫组织化学分析再生障碍性贫血（AA）、慢性髓性白血病（CML）患者及正常骨髓的骨髓活检标本，以确定造血干细胞（HSC）区室和骨髓微环境的变化。HSC水平在AA中最低，在CML中最高。T和B淋巴细胞在AA（p < 0.01）和CML（p < 0.01）中均减少。AA中观察到自然杀伤细胞，但CML和健康对照中未观察到（p < 0.01）。CML中未观察到巨噬细胞和肥大细胞。AA和CML的基质细胞成分存在显著差异。CML中未观察到巢蛋白细胞，基质细胞衍生因子-1阳性细胞的平均数量在CML中最低。骨桥蛋白细胞在AA中高于CML（p < 0.01）；骨连接蛋白细胞在CML中高于AA（p < 0.01）。AA和CML之间骨钙素的表达无显著差异。内皮细胞数量在CML中最高，在AA中最低（p < 0.01）。我们的研究结果表明，骨髓微环境成分的变化可能与导致AA和/或CML的造血功能缺陷有关。
