Even-Or Ehud, Eden-Walker Alexandra, Di Mola Maria, McDougall Elizabeth, Schechter Tal, Ali Muhammad, Svajger Gordana, Gassas Adam, Licht Christoph, Krueger Joerg
Division of Hematology/Oncology/BMT, Division of Nephrology, and the Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.
Transfusion. 2017 Jan;57(1):122-130. doi: 10.1111/trf.13859. Epub 2016 Oct 3.
Peripheral stem cell collections can be challenging in the pediatric population and respective experience is limited. Since February 2015 our institution is utilizing the new Spectra Optia (Optia) apheresis device, which has replaced the former COBE Spectra (COBE) device. As a quality initiative we collected and compared collection efficiency (CE2) and other collection variables between the two devices.
In this retrospective study we collected and compared clinical, laboratory, and technical collection data from stem cell collection procedures done with the Optia and COBE devices. The collected data included patient demographics, precollection peripheral CD34+ cell counts, total CD34+ cells collected, complete blood count, electrolytes before and after collection, side effects attributed to the collection, total blood volumes processed (TBVs), collection times, and calculated CE2 and collection ratios.
Forty-one collection procedures performed on 29 pediatric patients with the Optia device were compared to 41 collections performed on 27 patients with the COBE device. The TBVs through the Optia device were significantly smaller than the COBE (3.9 ± 0.2 × TBV vs. 5.5 ± 0.1 × TBV, respectively; p < 0.001), requiring significantly less anticoagulant and providing similar amounts of stem cells while collection times were significantly shorter (mean, 238 ± 9 min vs. 264 ± 9 min, respectively; p < 0.05). Collections on the Optia caused significantly smaller reductions of plasma calcium and magnesium. No significant side effects attributed to the procedure were noted.
Stem cell apheresis with the Optia device in children is safe and feasible with smaller blood volumes with shorter collection times.
在儿科人群中进行外周血干细胞采集具有挑战性，且相关经验有限。自2015年2月起，我们机构开始使用新型的Spectra Optia（Optia）血液成分分离设备，该设备已取代了之前的COBE Spectra（COBE）设备。作为一项质量改进举措，我们收集并比较了这两种设备的采集效率（CE2）及其他采集变量。
在这项回顾性研究中，我们收集并比较了使用Optia和COBE设备进行干细胞采集程序的临床、实验室及技术采集数据。收集的数据包括患者人口统计学信息、采集前外周血CD34+细胞计数、采集的CD34+细胞总数、全血细胞计数、采集前后的电解质、采集引起的副作用、处理的全血量（TBV）、采集时间，以及计算得出的CE2和采集比率。
将在29例儿科患者中使用Optia设备进行的41次采集程序与在27例患者中使用COBE设备进行的41次采集进行比较。通过Optia设备处理的全血量显著少于COBE设备（分别为3.9±0.2×TBV和5.5±0.1×TBV；p<0.001），所需抗凝剂显著减少，同时能采集到相似数量的干细胞，且采集时间显著缩短（分别为平均238±9分钟和264±9分钟；p<0.05）。使用Optia设备采集导致血浆钙和镁的降低幅度显著较小。未观察到该程序引起的显著副作用。
在儿童中使用Optia设备进行干细胞单采安全可行，所需血量较少且采集时间较短。
