Yamamoto Haru, Elbadawy Mohamed, Fujisaka Koudai, Sato Yomogi, Ohmori Takahiro, Shinohara Yuta, Hatano Yui, Kobayashi Daichi, Gomyo Ayana, Sudo Yuji, Azakami Daigo, Uchide Tsuyoshi, Fukushima Ryuji, Morita Shohei, Abugomaa Amira, Yamawaki Hideyuki, Kaneda Masahiro, Usui Tatsuya, Sasaki Kazuaki
Laboratory of Veterinary Pharmacology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt.
Animals (Basel). 2021 Sep 23;11(10):2770. doi: 10.3390/ani11102770.
In patients with solid tumors, circulating tumor cells (CTCs) spread in their blood and function as a seed for metastases. However, the study of CTCs has been limited by their rarity, low frequency, and heterogeneity. The efficient collection of CTCs will contribute to further research of metastatic cancers. Apheresis is a process in which the whole blood of an individual is passed through a machine that isolates a particular constituent and returns the remainder to the circulation. In the present study, we investigated the safety and feasibility of apheresis to separate peripheral blood monocytes (PBMCs), whose density is closely similar to that of CTCs, and to capture intravenously administered human breast cancer cells, MCF7s, from the dogs. No life-threatening events were observed in dogs during the apheresis process. The changes in the hemogram were transient and recovered gradually within a few days after apheresis. During apheresis, 50 mL of PBMCs could be collected from each dog. Notably, a thrombus was formed along the circuit wall during apheresis, which decreased the blood collection pressure. MCF7 cells were successfully captured by the apheresis machine. The captured cells were regrown in vitro and characterized compared with the original cells. In conclusion, apheresis could be safely performed in dogs to isolate CTCs with precautions to maintain hemodynamic stability.
在实体瘤患者中，循环肿瘤细胞（CTCs）在血液中扩散并作为转移的种子。然而，对CTCs的研究受到其稀有性、低频率和异质性的限制。CTCs的有效收集将有助于转移性癌症的进一步研究。血液成分单采术是一个过程，即个体的全血通过一台机器，该机器分离出特定成分并将其余部分返回循环系统。在本研究中，我们调查了血液成分单采术用于分离外周血单核细胞（PBMCs，其密度与CTCs的密度非常相似）以及从犬类中捕获静脉注射的人乳腺癌细胞MCF7s的安全性和可行性。在血液成分单采术过程中，未在犬类中观察到危及生命的事件。血常规的变化是短暂的，在血液成分单采术后几天内逐渐恢复。在血液成分单采术期间，每只犬可收集50 mL的PBMCs。值得注意的是，在血液成分单采术过程中，沿回路壁形成了血栓，这降低了采血压力。MCF7细胞被血液成分单采机成功捕获。捕获的细胞在体外重新生长，并与原始细胞进行表征比较。总之，在犬类中可以安全地进行血液成分单采术以分离CTCs，但需采取预防措施以维持血流动力学稳定性。
