Kitagawa Erina, Yamamoto Tatsuya, Fujishita Mayuko, Ota Yuki, Yamamoto Kohei, Nakagawa Tomoyuki, Hayakawa Takashi
a The United Graduate School of Agricultural Science , Gifu University , Gifu , Japan.
b Faculty of Health and Human Life , Nagoya Bunri University , Inazawa , Japan.
Biosci Biotechnol Biochem. 2017 Feb;81(2):316-322. doi: 10.1080/09168451.2016.1240604. Epub 2016 Oct 4.
We investigated the efficacy of supplementing the diet with choline or betaine in ameliorating lipid accumulation induced by vitamin B (B) deficiency in rat liver. Male Wistar rats were fed a control, B-deficient, choline-supplemented (2, 4, or 6 g choline bitartrate/kg diet) B-deficient diet or betaine-supplemented (1, 2, or 4 g betaine anhydrous/kg diet) B-deficient diet for 35 d; all diets contained 9 g L-methionine (Met)/kg diet. Choline or betaine supplementation attenuated liver lipid deposition and restored plasma lipid profiles to control levels. These treatments restored the disruptions in Met metabolism and the phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio induced by B deficiency in liver microsomes. These results suggest that choline and betaine ameliorated liver lipid accumulation induced by B deficiency via recovery of Met metabolism and very low-density lipoprotein secretion by restoring the supply of PC derived from PE.
我们研究了在大鼠肝脏中，通过饮食补充胆碱或甜菜碱来改善维生素B（B）缺乏引起的脂质蓄积的效果。将雄性Wistar大鼠分别喂食对照饮食、缺乏B的饮食、补充胆碱（2、4或6 g酒石酸胆碱/千克饮食）的缺乏B的饮食或补充甜菜碱（1、2或4 g无水甜菜碱/千克饮食）的缺乏B的饮食，持续35天；所有饮食均含有9 g L-蛋氨酸（Met）/千克饮食。补充胆碱或甜菜碱可减轻肝脏脂质沉积，并使血浆脂质谱恢复至对照水平。这些处理恢复了肝脏微粒体中由B缺乏引起的Met代谢紊乱以及磷脂酰胆碱（PC）/磷脂酰乙醇胺（PE）比值。这些结果表明，胆碱和甜菜碱通过恢复Met代谢以及通过恢复由PE衍生的PC供应来改善极低密度脂蛋白分泌，从而改善了由B缺乏引起的肝脏脂质蓄积。
