Koloren Zeynep, Seferoğlu Onuralp, Karanis Panagiotis
Ordu University, Faculty of Arts and Sciences, Department of Biology, Ordu,Turkey.
Ordu University, Faculty of Arts and Sciences, Department of Biology, Ordu,Turkey.
Acta Trop. 2016 Dec;164:337-344. doi: 10.1016/j.actatropica.2016.09.025. Epub 2016 Sep 30.
A total of 420 environmental water samples and 120 drinking water samples from 45 different sampling sites of the Black Sea in Turkey were collected between 2012 and 2014. Genomic DNA was isolated from all the investigated water samples and comparativelly analyzed by Loop-mediated isothermal amplification (LAMP) of the elongation factor 1 Alfa (EF1α) gene, and by nested Polymerase Chain Reaction (nPCR) of the small subunit (SSU) rRNA and semi-nested PCR (snPCR) of the glutamate dehydrogenase gene (GDH). 141 (58.7%), 125 (52.1%) and 120 (50%) samples respectivelly were positive by each method. Out of 240 environmental samples collected from 25 sites of Samsun Province have been found positive for G. duodenalis by LAMP, nPCR and snPCR, respectively. 55 (30.5%), 50 (27.8%) and 47 (26.1%) of 180 environmental samples collected from 20 other sampling sites of Giresun Province were positive for Giardia by LAMP, nPCR and snPCR, respectively. Five PCR products from different samples of the Giresun Province and 10 other samples from the Samsun Province were found positive for G. duodenalis assemblage B. Five PCR products from Giresun Province and 5 samples from Samsun Province were found positive for G. duodenalis assemblage A. This is the first report about G. duodenalis assemblages A and B from water samples investigations in Black Sea of Turkey.
2012年至2014年期间，从土耳其黑海45个不同采样点共采集了420份环境水样和120份饮用水样。从所有调查水样中提取基因组DNA，并通过延伸因子1阿尔法（EF1α）基因的环介导等温扩增（LAMP）、小亚基（SSU）rRNA的巢式聚合酶链反应（nPCR）和谷氨酸脱氢酶基因（GDH）的半巢式PCR（snPCR）进行比较分析。每种方法分别有141份（58.7%）、125份（52.1%）和120份（50%）样本呈阳性。在从萨姆松省25个地点采集的240份环境样本中，分别通过LAMP、nPCR和snPCR检测出十二指肠贾第虫呈阳性。在从吉雷松省其他20个采样点采集的180份环境样本中，分别有55份（30.5%）、50份（27.8%）和47份（26.1%）通过LAMP、nPCR和snPCR检测出贾第虫呈阳性。来自吉雷松省不同样本的5个PCR产物和来自萨姆松省的10个其他样本被检测出十二指肠贾第虫B群呈阳性。来自吉雷松省的5个PCR产物和来自萨姆松省的5个样本被检测出十二指肠贾第虫A群呈阳性。这是关于土耳其黑海水样调查中十二指肠贾第虫A群和B群的首次报告。
