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人脐带间充质干细胞移植治疗实验性脑卒中后梗死周边区的蛋白质组学分析

Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke.

作者信息

Dongsheng He, Zhuo Zhang, Jiamin Lao, Hailan Meng, Lijuan Han, Fan Chen, Dan Ye, He Zhang, Yun Xu

机构信息

1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.

4Department of Gastroenterology, Children's Hospital of Nanjing, Nanjing Medical University, Nanjing 210008, China.

出版信息

Aging Dis. 2016 Oct 1;7(5):623-634. doi: 10.14336/AD.2016.0121. eCollection 2016 Oct.

DOI:10.14336/AD.2016.0121
PMID:27699085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5036957/
Abstract

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

摘要

在中风的各种治疗方法中，用人脐带间充质干细胞（hUC-MSCs）进行治疗已取得了一些有前景的结果。然而，其潜在机制仍不清楚。我们分析了缺血性中风后用hUC-MSCs治疗的皮质梗死周边区域的蛋白质表达谱，发现在接受或未接受hUC-MSCs治疗的组之间有16种蛋白质表达存在差异。这些蛋白质通过基因本体术语分析以及与CD200-CD200R1、CCL21-CXCR3和转录因子的网络进一步确定。其中三种：Abca13、Grb2和Ptgds通过qPCR和ELISA进行了验证。我们发现Abca13的蛋白质水平和Grb2的mRNA水平与蛋白质组学分析结果一致。最后，描述了这些蛋白质的功能，并强调了值得进一步研究的潜在蛋白质。我们的数据可能为使用hUC-MSCs治疗中风提供潜在机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/68b5a242fcc3/ad-7-5-623-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/4d612865641a/ad-7-5-623-g1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/3f1705db7e16/ad-7-5-623-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/59efb70fb3e7/ad-7-5-623-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/68b5a242fcc3/ad-7-5-623-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/4d612865641a/ad-7-5-623-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/01829ffd7d1f/ad-7-5-623-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/3f1705db7e16/ad-7-5-623-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/59efb70fb3e7/ad-7-5-623-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b18a/5036957/68b5a242fcc3/ad-7-5-623-g5.jpg

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Genome-wide transcriptional profiling analysis reveals annexin A6 as a novel EZH2 target gene involving gastric cellular proliferation.
Identification of Novel Circulating miRNAs in Patients with Acute Ischemic Stroke.
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