Adachi H
Nihon Hoigaku Zasshi. 1989 Feb;43(1):27-39.
The appearance rate of Y chromatin was calculated in the dental pulp with the staining by Quinacrine Mustard. The morphological findings of the tissue were observed with the HE stained section and the change of the appearance rate of Y chromatin was studied along with the morphological change of the tissue. The average of the appearance rate of Y chromatin in the male dental pulp was 42.2% immediately after the tooth extraction, 34.8% after one month, 27.9% after 3 months, 20.8% after 6 months, 20.6% after 1 year, 19.1% after 18 months and 11.4% after 2 years, respectively. On the other hand, the appearance rate of the pseudo-Y chromatin spot in the female pulp was below 4.2% through the two years' observation which enabled to determine the sexuality. By the HE staining observation about the dental pulp, left at a room temperature, it was revealed that the pulp tissue can be well preserved for a long time because of the natural drying. The degree of the staining declined with the lapse of time after the removal of tooth. Degeneration of tissue did not offer any discrete criteria for the estimation of the lapse of time after the removal of tooth.
用喹吖因芥染色法计算牙髓中Y染色质的出现率。用苏木精-伊红（HE）染色切片观察组织的形态学表现，并研究Y染色质出现率随组织形态学变化的情况。男性牙髓中Y染色质出现率的平均值在拔牙后立即为42.2%，1个月后为34.8%，3个月后为27.9%，6个月后为20.8%，1年后为20.6%，18个月后为19.1%，2年后为11.4%。另一方面，在两年的观察中，女性牙髓中假Y染色质点的出现率低于4.2%，这使得能够确定性别。通过对室温下保存的牙髓进行HE染色观察发现，由于自然干燥，牙髓组织可以长时间保存良好。拔牙后随着时间的推移，染色程度下降。组织变性并未为估计拔牙后时间的推移提供任何明确的标准。
