Kuramoto Hiroyuki, Iwami Yoshiko, Sugimoto Naoko, Miyagawa Jun, Banno Midori, Iida Manichi
Department of Cancer Detection for Females, The Kanagawa Health Service Association, Yokohama, Japan.
Acta Cytol. 2016;60(5):429-437. doi: 10.1159/000448872. Epub 2016 Oct 5.
To evaluate whether or not the liquid-based procedure (LBP) for endometrial cytology is as worthwhile for endometrial phasing as conventional slides.
The subjects were 81 women who underwent endometrial cytology and were defined as negative. The specimens obtained by either Endocyte® or Masubuchi aspiration tube® were processed first with the conventional procedure and then with LBP using TACAS™.
(1) The number of subjects diagnosed by the conventional method as having proliferative, mid-, middle-secretory and late-secretory and atrophic phases was 40, 11, 10, 0 and 20, respectively. The rate of agreement with those using LBP was 87.7%. (2) Incidences of large clusters, ductal clusters, palisade arrangement, uneven staining and dirty mucous background detected were significantly higher with the conventional method, whereas with LBP clean background, inconspicuous bonding of cells, scattered solitary glandular cells, clear well-stained cytoplasm and cell compactness were higher. (3) Especially in the proliferative phase, clusters tended to be smaller and lose their architectural structures, and scattered solitary columnar cells were present. (4) Cells in the mid-phase tended to have loose contact and to mimic other phases.
Cytodiagnosis of endometrial phasing prepared with LBP is feasible to perform when some modifications are implemented.
评估子宫内膜细胞学的液基制片法（LBP）在子宫内膜分期方面是否与传统涂片一样有价值。
研究对象为81例行子宫内膜细胞学检查且结果为阴性的女性。通过Endocyte®或益渊吸引管®获取的标本，先采用传统方法处理，然后使用TACAS™进行液基制片法处理。
（1）传统方法诊断为增殖期、中期、中分泌期、晚分泌期和萎缩期的患者人数分别为40、11、10、0和20。与液基制片法诊断结果的一致率为87.7%。（2）传统方法检测到的大细胞团、导管样细胞团、栅栏状排列、染色不均和脏污黏液背景的发生率显著更高，而液基制片法的背景干净、细胞黏附不明显、散在孤立腺细胞、细胞质染色清晰且细胞致密程度更高。（3）尤其是在增殖期，细胞团往往较小且失去其结构，存在散在的孤立柱状细胞。（4）中期细胞往往接触松散，易与其他期混淆。
对液基制片法制备的子宫内膜分期进行细胞诊断，在进行一些改进后是可行的。
