溶壁微球菌（藤黄微球菌）对数期细胞中的细胞壁自溶作用。
Cell wall autolysis in log phase cells of Micrococcus lysodeikticus (luteus).
作者信息
Monodane T, Matsushima Y, Hirachi Y, Kotani S
出版信息
Microbiol Immunol. 1978;22(2):57-66. doi: 10.1111/j.1348-0421.1978.tb00349.x.
Abstract
Log phase cells of Micrococcus lysodeikticus (luteus) IFO 3333 autolyzed when incubated at 37 C in 0.01 M sodium-phosphate buffer pH 7.5. The enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an N-acetylmuramyl-L-alanine amidase. The autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer.
摘要
溶壁微球菌（藤黄微球菌）IFO 3333的对数期细胞在37℃于pH 7.5的0.01M磷酸钠缓冲液中孵育时会发生自溶。参与自溶的酶主要从用正丁醇处理过的细胞质膜和细胞质物质的水相中回收，并且被证明是一种N-乙酰胞壁酰-L-丙氨酸酰胺酶。向自溶缓冲液中添加胰蛋白酶会抑制悬浮在该缓冲液中的对数期细胞的自溶。
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