Guan X, Su M C, Zhao R B, Ouyang H M, Dong X D, Hu P, Pei Q, Lu J, Li Z F, Zhang C R, Yang T-H
Department of Hematology, the First People's Hospital of Yunnan Province, Kunming, China.
Bifeng Technology Co., Ltd. of Guangdong Province, Zhuhai, China 3Department of Clinical Laboratory, The First People's Hospital of Yunnan Province, Kunming, China.
Genet Mol Res. 2016 Aug 19;15(3):gmr7662. doi: 10.4238/gmr.15037662.
Cinnamon is the main component of Sanyangxuedai, which is one of the effective traditional Chinese medicines for treating malignancies. Leukemia is a prevalent malignant disease that Sanyangxuedai has been used to treat. Although successful in several studies, there is a lack of solid evidence as to why Sanyangxuedai has an effect on leukemia, and little is known about the underlying mechanisms. In this study, the active ingredients of cinnamon were isolated, purified, and identified. The transwell transport pool formed with the Caco-2 cell model was used to filter the active ingredients of cinnamon by simulating the gastrointestinal barrier in vitro. Moreover, the cell morphology, cell cycle status, apoptosis status, and antigenic variation of the cell surface antigens were observed and measured in K562 cells after treatment with the active ingredients of cinnamon. Our results showed that 50-75 μM was a safe concentration of cinnamon extract for treatment of K562 cells for 72 h. The cinnamon extract caused growth inhibition of K562 cells. Cinnamon extract seemed to arrest the cells at the G1 stage and increased the apoptosis rate significantly. Interestingly, cinnamon extract treatment upregulated the expression of erythroid and myeloid differentiation antigens and downregulated that of the megakaryocytic differentiation antigens in a dose-dependent manner. Our findings indicate that cinnamon extract from Sanyangxuedai may be effective for treating leukemia.
肉桂是三阳血傣的主要成分，三阳血傣是治疗恶性肿瘤的有效传统中药之一。白血病是一种常见的恶性疾病，三阳血傣一直被用于治疗该病。尽管在一些研究中取得了成功，但关于三阳血傣为何对白血病有疗效缺乏确凿证据，其潜在机制也知之甚少。在本研究中，对肉桂的活性成分进行了分离、纯化和鉴定。利用Caco - 2细胞模型形成的跨膜转运池在体外模拟胃肠道屏障来筛选肉桂的活性成分。此外，在用肉桂活性成分处理K562细胞后，观察并检测了细胞形态、细胞周期状态、凋亡状态以及细胞表面抗原的抗原性变化。我们的结果表明，50 - 75μM是肉桂提取物处理K562细胞72小时的安全浓度。肉桂提取物导致K562细胞生长抑制。肉桂提取物似乎使细胞停滞在G1期，并显著提高凋亡率。有趣的是，肉桂提取物处理以剂量依赖的方式上调红系和髓系分化抗原的表达，下调巨核系分化抗原的表达。我们的研究结果表明，三阳血傣中的肉桂提取物可能对治疗白血病有效。
