Jabri C, Sbei H, Zitouna N, Trifi-Farah N, Zoghlami Khelil A
Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences, University of Tunis El Manar, Tunis, Tunisia.
Laboratory of Plant Breeding, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Genet Mol Res. 2016 Aug 26;15(3):gmr8595. doi: 10.4238/gmr.15038595.
Medicago ciliaris L., considered as a valuable genetic resource, is a good candidate for the improvement of marginal or degraded lands with low fertility or high salinity. In this study, the pheno-morphological and genetic diversity were investigated in 14 Tunisian populations of M. ciliaris for the first time. Fourteen morphological traits showed significant differentiation between populations and high levels of diversity. Two amplified fragment length polymorphism primer combinations (E-AGC/M-CAA; E-AAG/M-CTG) were analyzed using an automated capillary electrophoresis system. A total of 528 loci were generated, of which 54% were polymorphic. Allelic polymorphism ranged from 0.02 to 0.5. Significant variation between populations was found for gene diversity, mean number of alleles per locus and Shannon index for which mean values were 0.17, 0.26, and 1.57, respectively. Analysis of molecular variance revealed a high rate of genetic variation within populations. Principal component analysis and genotypic clustering discriminated M. ciliaris populations according to their geographical origin. M. ciliaris clustered into three main groups. The first group was associated with high inland and cold areas, the second was defined by low areas with mild winters while the third described low coastal areas. Similarity of morphological and molecular results indicated that either markers could be used for the study of genetic diversity in this species.
紫花苜蓿被认为是一种宝贵的遗传资源，是改良肥力低或盐度高的边缘或退化土地的良好候选植物。在本研究中，首次对突尼斯的14个紫花苜蓿种群进行了表型形态和遗传多样性调查。14个形态性状在种群间表现出显著差异和高水平的多样性。使用自动毛细管电泳系统分析了两种扩增片段长度多态性引物组合（E-AGC/M-CAA；E-AAG/M-CTG）。共产生了528个位点，其中54%为多态性位点。等位基因多态性范围为0.02至0.5。在基因多样性、每个位点的平均等位基因数和香农指数方面，种群间存在显著差异，其平均值分别为0.17、0.26和1.57。分子方差分析显示种群内遗传变异率较高。主成分分析和基因型聚类根据其地理起源区分了紫花苜蓿种群。紫花苜蓿分为三个主要组。第一组与内陆高海拔寒冷地区相关，第二组由冬季温和的低海拔地区定义，第三组描述的是沿海低海拔地区。形态学和分子结果的相似性表明，这两种标记都可用于该物种遗传多样性的研究。
