Song F S, Ni J L, Qian Y L, Li L, Ni D H, Yang J B
Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China.
Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China.
Genet Mol Res. 2016 Aug 29;15(3):gmr8512. doi: 10.4238/gmr.15038512.
Molecular markers can increase both the efficiency and speed of breeding programs. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. In this study, we used newly derived cleaved amplified polymorphic sequence markers to detect the functional mutations of tms5, which is a male sterile gene that is widely used in rice production in China. In addition, restriction cutting sites were designed to specifically digest amplicons of tms5 but not wild type (TMS5), in order to avoid the risk of false positive results. By optimizing the condition of the polymerase chain reaction amplifications and restriction enzyme digestions, the newly designed markers could accurately distinguish between tms5 and TMS5. These markers can be applied in marker-assisted selection for breeding novel thermo-sensitive genic male sterile (TGMS) lines, as well as to rapidly identify the TGMS hybrid seed purity.
分子标记可以提高育种计划的效率和速度。检测导致表型变化的功能突变的功能标记为基因鉴定提供了一种精确的方法。在本研究中，我们使用新衍生的酶切扩增多态性序列标记来检测tms5的功能突变，tms5是一种在中国水稻生产中广泛使用的雄性不育基因。此外，设计了限制性切割位点，以特异性消化tms5的扩增子，而不消化野生型（TMS5），以避免假阳性结果的风险。通过优化聚合酶链反应扩增和限制性酶切的条件，新设计的标记可以准确区分tms5和TMS5。这些标记可应用于标记辅助选择，以培育新型温敏核雄性不育（TGMS）系，以及快速鉴定TGMS杂交种子纯度。
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