Yang Qingkai, Liang Chunyang, Zhuang Wen, Li Jun, Deng Huabing, Deng Qiyun, Wang Bin
The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Planta. 2007 Jan;225(2):321-30. doi: 10.1007/s00425-006-0353-6. Epub 2006 Aug 2.
Previous research has demonstrated that the thermo-sensitive genic male-sterile (TGMS) gene in rice was regulated by temperature. TGMS rice is important to hybrid rice production because the application of the TGMS system in two-line breeding is cost-effective, simple, efficient and overcomes the limitations of the cytoplasmic male sterility (CMS) system. AnnongS is the first discovered and deeply studied TGMS rice line in China. Previous studies have suggested that AnnongS-1 and Y58S, two derivative TGMS lines of AnnongS, were both controlled by a single recessive gene named tms5, which was genetically mapped on chromosome 2. In the current study, three populations (AnnongS-1 x Nanjing11, Y58S x Q611, and Y58S x Guanghui122) were developed to investigate the tms5 gene molecular map. Analysis of recombination events of sterile samples, utilizing 125 probes covering the tms5 region, suggested that the tms5 gene was physically mapped to a 19 kb DNA fragment between two markers, 4039-1 and 4039-2, located on the BAC clone AP004039. Following the construction of a physical map between the two markers, ONAC023, a member of the NAC (NAM-ATAF-CUC-related) gene family, was identified as the candidate of the tms5 gene.
先前的研究表明，水稻中的温敏雄性不育（TGMS）基因受温度调控。TGMS水稻对杂交水稻生产很重要，因为TGMS系统在两系育种中的应用具有成本效益、简单高效，且克服了细胞质雄性不育（CMS）系统的局限性。安农S是中国首个被发现并深入研究的TGMS水稻品系。先前的研究表明，安农S的两个衍生TGMS品系安农S-1和Y58S均受一个名为tms5的单隐性基因控制，该基因在遗传上定位在2号染色体上。在本研究中，构建了三个群体（安农S-1×南京11、Y58S×Q611和Y58S×广恢122）来研究tms5基因的分子图谱。利用覆盖tms5区域的125个探针分析不育样本的重组事件，结果表明tms5基因在物理上定位在位于BAC克隆AP004039上的两个标记4039-1和4039-2之间的一个19 kb DNA片段上。在构建了两个标记之间的物理图谱后，NAC（NAM-ATAF-CUC相关）基因家族的成员ONACo23被鉴定为tms5基因的候选基因。
