Sheng J Q, Shu Q C, Shi J W, Wang J H, Peng K, Yuan S, Hong Y J
Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China
Genet Mol Res. 2016 Aug 29;15(3):gmr8533. doi: 10.4238/gmr.15038533.
Ferritin is a conserved iron-binding protein involved in host defense and cellular iron metabolism in most organisms. We investigated the expression profiles of two ferritin genes (designated HsFer-1 and HsFer-2) in the hemocytes, gonad, and hepatopancreas of Hyriopsis schlegelii, when challenged with bacteria and metal ions. HsFer gene transcription increased 1.8-7.7- and 1.9-6.1-fold in these tissues after stimulation with Staphylococcus aureus and Vibrio anguillarum, respectively. In addition, following exposure to Fe, expression of HsFer-1 and HsFer-2 was elevated by 1.5-6.1- and 3.6-10.1-fold, respectively. Levels of HsFer-1 and -2 mRNA also increased significantly after treatment with Cu and Pb at certain concentrations. Moreover, recombinant HsFer-1 and -2 were able to inhibit the growth of two strains of bacteria, and the former efficiently chelated Fe. From these results, we conclude that HsFer-1 and -2 may be involved in iron metabolism and immune defense by inhibiting the growth of bacteria.
铁蛋白是一种保守的铁结合蛋白，在大多数生物体中参与宿主防御和细胞铁代谢。我们研究了在受到细菌和金属离子刺激时，中华圆田螺血细胞、性腺和肝胰腺中两个铁蛋白基因（命名为HsFer - 1和HsFer - 2）的表达谱。在用金黄色葡萄球菌和鳗弧菌刺激后，这些组织中HsFer基因转录分别增加了1.8 - 7.7倍和1.9 - 6.1倍。此外，暴露于铁后，HsFer - 1和HsFer - 2的表达分别提高了1.5 - 6.1倍和3.6 - 10.1倍。在一定浓度的铜和铅处理后，HsFer - 1和 - 2的mRNA水平也显著增加。此外，重组HsFer - 1和 - 2能够抑制两株细菌的生长，并且前者能有效螯合铁。从这些结果来看，我们得出结论，HsFer - 1和 - 2可能通过抑制细菌生长参与铁代谢和免疫防御。
