Su W T, Liu Y J, Huang T Y
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
Genet Mol Res. 2016 Aug 30;15(3):gmr8993. doi: 10.4238/gmr.15038993.
Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whereas no such aggregation was observed in cells cultured on 24-well plates. Hepatocyte spheroids formed on the PLA-CS nanofibers displayed excellent hepatic-related protein expression, such as albumin and urea, compared to HepG2 cells cultured on the 24-well plates. These results indicated that formation of the hepatocyte spheroids in nanofibers can increase and maintain hepatocyte functions for a longer time, supporting a new strategy for bioartificial liver development.
肝细胞球体的形成是在体外增强肝脏特异性功能的必要策略。在本研究中，HepG2细胞在聚乳酸-壳聚糖（PLA-CS）纳米纤维上生长时显示出良好的活力，并在培养5天后在PLA-CS纳米纤维上聚集形成直径约100-200μm的多细胞球体，而在24孔板上培养的细胞中未观察到这种聚集现象。与在24孔板上培养的HepG2细胞相比，在PLA-CS纳米纤维上形成的肝细胞球体表现出优异的肝脏相关蛋白表达，如白蛋白和尿素。这些结果表明，纳米纤维中肝细胞球体的形成可以在更长时间内增强和维持肝细胞功能，为生物人工肝的发展提供了一种新策略。
