Nacheva G A, Preobrazhenskaia O V, Mel'nikova A F, Karpov V L
Mol Biol (Mosk). 1989 May-Jun;23(3):879-88.
Using "protein-image" hybridization technique combined with various crosslinking methods, for formaldehyde-prefixed nuclei we have analysed changes induced by activation in the chromatin structure of HSP-70 genes. From the crosslinking data it follows that chromatin of actively transcribed genes undergoes some structural rearrangements resulting in certain weakening of the contacts between DNA and the globular parts of histones so that the histones remain bound to DNA through their N-terminal regions. In addition, there have been found two specific regions with a reduced content of histones: the 5'-promoter of HSP-70 gene and a region distanced by approximately 1 k.b. from the 3'-end of the HSP-70 gene.
运用“蛋白质-图像”杂交技术并结合多种交联方法，对于经甲醛预处理的细胞核，我们分析了热休克蛋白70（HSP-70）基因染色质结构激活所诱导的变化。从交联数据可知，活跃转录基因的染色质会经历一些结构重排，导致DNA与组蛋白球状部分之间的某些接触减弱，从而使组蛋白通过其N端区域与DNA保持结合。此外，还发现了两个组蛋白含量降低的特定区域：HSP-70基因的5'启动子以及距HSP-70基因3'端约1千碱基处的一个区域。
