Hrubisko M, Kysela B, Kuliffay P
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Neoplasma. 1989;36(4):401-10.
Growth, flow cytometric, and karyological characteristics were analyzed with respect to differences between sensitive L1210 cells and eight sublines resistant to cisplatin (DDP), 1,2-diaminocyclohexane(DACH)-Pt(II) citrate, DACH-Pt(II) glucarate, cis-dichloro-bis-(isopropylamine)-trans-dihydroxyplatinum (IV) (CHIP, iproplatin), and methotrexate (MTX). No great differences were found in growth properties. The sensitive and a majority of resistant sublines displayed similar flow cytometric and karyological characteristics, major differences were found only in the sublines resistant to CHIP. The relationship between the mechanism(s) of resistance and different pharmacokinetics of the drugs is discussed with special reference to DNA content and chromosome structure.
针对敏感的L1210细胞与八个对顺铂（DDP）、柠檬酸1,2 - 二氨基环己烷（DACH）-Pt(II)、葡糖二酸DACH - Pt(II)、顺 - 二氯 - 双 -（异丙胺）-反 - 二羟基铂（IV）（CHIP，异丙铂）和甲氨蝶呤（MTX）耐药的亚系之间的差异，分析了生长、流式细胞术和染色体学特征。在生长特性方面未发现显著差异。敏感细胞系和大多数耐药亚系表现出相似的流式细胞术和染色体学特征，仅在对CHIP耐药的亚系中发现了主要差异。结合DNA含量和染色体结构，特别讨论了耐药机制与药物不同药代动力学之间的关系。
