小鼠磷脂氢过氧化物谷胱甘肽过氧化物酶4的晶体结构和溶液结构研究
Crystal and solution structural studies of mouse phospholipid hydroperoxide glutathione peroxidase 4.
作者信息
Janowski Robert, Scanu Sandra, Niessing Dierk, Madl Tobias
机构信息
Group Intracellular Transport and RNA Biology, Institute of Structural Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Institute of Structural Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
出版信息
Acta Crystallogr F Struct Biol Commun. 2016 Oct 1;72(Pt 10):743-749. doi: 10.1107/S2053230X16013686. Epub 2016 Sep 22.
The mammalian glutathione peroxidase (GPx) family is a key component of the cellular antioxidative defence system. Within this family, GPx4 has unique features as it accepts a large class of hydroperoxy lipid substrates and has a plethora of biological functions, including sperm maturation, regulation of apoptosis and cerebral embryogenesis. In this paper, the structure of the cytoplasmic isoform of mouse phospholipid hydroperoxide glutathione peroxidase (O70325-2 GPx4) with selenocysteine 46 mutated to cysteine is reported solved at 1.8 Å resolution using X-ray crystallography. Furthermore, solution data of an isotope-labelled GPx protein are presented.
哺乳动物谷胱甘肽过氧化物酶（GPx）家族是细胞抗氧化防御系统的关键组成部分。在这个家族中，GPx4具有独特的特征，因为它能接受一大类氢过氧脂质底物，并且具有多种生物学功能，包括精子成熟、细胞凋亡调节和大脑胚胎发育。本文报道了通过X射线晶体学以1.8 Å分辨率解析的小鼠磷脂氢过氧化物谷胱甘肽过氧化物酶（O70325-2 GPx4）胞质异构体的结构，其中第46位硒代半胱氨酸突变为半胱氨酸。此外，还给出了同位素标记的GPx蛋白的溶液数据。
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