2011 - 2013年约旦甲型（H1N1）pdm09和H3N2病毒的分子流行病学及进化

Molecular epidemiology and evolution of A(H1N1) pdm09 and H3N2 viruses in Jordan, 2011-2013.

作者信息

El Shesheny R, Halasa N B, Williams J V, Shehabi A A, Faouri S, Kayali G, Khuri-Bulos N

机构信息

Centre of Excellence for Influenza Viruses, National Research Centre, Giza, Egypt.

Department of Pediatrics, Vanderbilt University, Nashville, Tennessee, United States of America.

出版信息

East Mediterr Health J. 2016 Oct 2;22(7):491-502.

PMID:27714744

Abstract

Understanding the genetic evolution of A(H1N1)pdm09 and H3N2 viruses can help better select strains to be included in the annual influenza vaccine. There is little information on their evolution in Jordan so this study investigated the genetic and antigenic variability of A(H1N1)pdm09 and H3N2 viruses in Jordan by performing phylogenetic and genetic analyses of the HA and NA genes of A(H1N1)pdm09 and H3N2 viruses between 2011 and 2013. The full HA and NA genes of 16 H1N1-positive samples obtained in our study and 21 published HA sequences and 20 published NA sequences from Jordanian viruses that were available on online gene databases were analysed. For H3N2, we generated 20 HA and 19 NA sequences and included 19 published HA and NA sequences each in the analysis. Jordanian H1N1 viruses had mutations that are characteristic of antigenic group 6 while H3N2 virus mutations belonged to group 3. No markers of resistance to oseltamivir were detected. The individual mutations are described in detail.

摘要

了解甲型（H1N1）pdm09和H3N2病毒的基因进化有助于更好地选择年度流感疫苗中包含的毒株。关于它们在约旦的进化情况，所知信息甚少，因此本研究通过对2011年至2013年间甲型（H1N1）pdm09和H3N2病毒的血凝素（HA）和神经氨酸酶（NA）基因进行系统发育和遗传分析，调查了约旦甲型（H1N1）pdm09和H3N2病毒的遗传和抗原变异性。分析了我们研究中获得的16份H1N1阳性样本的完整HA和NA基因，以及在线基因数据库中可获取的来自约旦病毒的21条已发表的HA序列和20条已发表的NA序列。对于H3N2，我们生成了20条HA序列和19条NA序列，并在分析中分别纳入了19条已发表的HA和NA序列。约旦的H1N1病毒具有抗原6组的特征性突变，而H3N2病毒的突变属于3组。未检测到对奥司他韦耐药的标志物。详细描述了各个突变情况。