Lala A K, Koppaka V
Photochem Photobiol. 1989 Jun;49(6):763-7. doi: 10.1111/j.1751-1097.1989.tb05574.x.
Fluorescence quenching technique has been used to determine the transverse location of the fluorescent fluorenyl fatty acids in single bilayer vesicles prepared from phosphatidylcholine. The fluorenyl fatty acids used here are 2-fluorenyl acetic, butyric, hexanoic and octanoic acid. In addition a new type of fluorescent probe, 7-n-butyl-fluorene-2-butyric acid, wherein a hydrophobic tail is attached to 2-fluorenyl-butyric acid has also been used to study its effect on alignment of these probes in the membrane. The association properties of the quencher 9,10-dibromostearic acid have been analysed. It is observed that the quencher association involves partitioning into the vesicles and does not involve any binding to the vesicles. The absolute partition coefficient of the 9,10-dibromostearic acid which partitions between the aqueous and the lipid phases of the phospholipid dispersion has been evaluated. Using this information the corrected Stern-Volmer plots were drawn and the bimolecular quenching constant evaluated.
荧光猝灭技术已被用于确定由磷脂酰胆碱制备的单层囊泡中荧光芴基脂肪酸的横向位置。这里使用的芴基脂肪酸是2-芴基乙酸、丁酸、己酸和辛酸。此外，一种新型荧光探针，7-正丁基芴-2-丁酸，其中疏水尾连接到2-芴基丁酸上，也被用于研究其对这些探针在膜中排列的影响。分析了猝灭剂9,10-二溴硬脂酸的缔合性质。观察到猝灭剂缔合涉及分配到囊泡中，而不涉及与囊泡的任何结合。评估了在磷脂分散体的水相和脂质相之间分配的9,10-二溴硬脂酸的绝对分配系数。利用这些信息绘制了校正后的斯特恩-沃尔默图，并评估了双分子猝灭常数。
