Henriksen-Lacey Malou, Bramwell Vincent, Perrie Yvonne
School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK.
Pharmaceutics. 2010 Mar 31;2(2):91-104. doi: 10.3390/pharmaceutics2020091.
A relatively simple and effective method to follow the movement of pharmaceutical preparations such as vaccines in biodistribution studies is to radiolabel the components. Whilst single radiolabelling is common practice, in vaccine systems containing adjuvants the ability to follow both the adjuvant and the antigen is favourable. To this end, we have devised a dual-radiolabelling method whereby the adjuvant (liposomes) is labelled with ³H and the antigen (a subunit protein) with I. This model is stable and reproducible; we have shown release of the radiolabels to be negligible over periods of up to 1 week in foetal calf serum at 37 ºC. In this paper we describe the techniques which enable the radiolabelling of various components, assessing stability and processing of samples which all for their application in biodistribution studies. Furthermore we provide examples derived from our studies using this model in tuberculosis vaccine biodistribution studies.
在生物分布研究中，一种相对简单且有效的追踪疫苗等药物制剂移动的方法是对其成分进行放射性标记。虽然单放射性标记是常见做法，但在含有佐剂的疫苗系统中，能够同时追踪佐剂和抗原是很有利的。为此，我们设计了一种双放射性标记方法，即佐剂（脂质体）用³H标记，抗原（一种亚单位蛋白）用I标记。该模型稳定且可重复；我们已表明，在37℃的胎牛血清中，长达1周的时间内放射性标记的释放可忽略不计。在本文中，我们描述了实现各种成分放射性标记的技术，评估了样品的稳定性和处理过程，这些都适用于生物分布研究。此外，我们还提供了在结核病疫苗生物分布研究中使用该模型的研究示例。
