Grekas D, Kalekou H, Tourkantonis A
First Medical Department, University Hospital AHEPA, Thessaloniki, Greece.
Ren Fail. 1989;11(1):27-31. doi: 10.3109/08860228909066943.
Six healthy dogs, after control measurements, were subjected to renal artery occlusion for 90 min, under general anesthesia. 5 mg of PGE2 (Prostin E2, Upjohn) diluted in 100 mL of normal saline was infused directly into the left renal artery for 90 min (PG group), while only normal saline was infused into the right renal artery (control group). After removal of the clamps blood and urine samples were collected every hour for the first 3 h of revascularization and both kidneys were then removed for histological study by light microscopy. PG kidneys regained urine flow above the oliguric levels within 3 hr of revascularization (0.30 +/- 0.04 mL/min vs 0.14 +/- 0.03 mL/min in controls (p less than 0.001). Creatinine, urea, and osmolar clearances were also significantly higher in PG group (p less than 0.01 or p less than 0.001 for 2 and 3 hr of follow-up after revascularization). Urine sodium concentration and fractional sodium excretion (FENa) (%) were not significantly lower in the PG group compared to the controls. Histology showed focal brushborder loss, hyaline and granular casts, focal tubular dilation, and focal necrotic lesions on the tubular cells in both groups of kidneys. A direct cytoprotective effect of the PGE2 was not found. It is suggested that PGE2 is effective in the protection of renal function when it is administered during the mechanical occlusion model of acute renal failure in anesthetized dogs.
六只健康犬在进行对照测量后，在全身麻醉下接受肾动脉阻断90分钟。将5毫克前列腺素E2（Prostin E2，Upjohn）稀释于100毫升生理盐水中，直接注入左肾动脉90分钟（PG组），而右肾动脉仅注入生理盐水（对照组）。去除血管夹后，在血管再通的前3小时每小时采集血液和尿液样本，然后切除双侧肾脏，进行光学显微镜组织学研究。PG组肾脏在血管再通后3小时内恢复到少尿水平以上的尿流（0.30±0.04毫升/分钟，而对照组为0.14±0.03毫升/分钟，p<0.001）。PG组肌酐、尿素和渗透清除率也显著更高（血管再通后随访2小时和3小时，p<0.01或p<0.001）。PG组尿钠浓度和钠排泄分数（FENa）（%）与对照组相比无显著降低。组织学显示两组肾脏均有局灶性刷状缘丧失、透明和颗粒管型、局灶性肾小管扩张以及肾小管细胞的局灶性坏死病变。未发现前列腺素E2有直接的细胞保护作用。提示在麻醉犬急性肾衰竭的机械性阻断模型中，前列腺素E2在肾功能保护方面是有效的。
