Ayanur Anjaneya, Singh Karam Pal, Cherian Susan, Singh Vidya, Nandi Sukdeb, Saminathan Mani, Jaynudin Khorajiya, SinghYadav Jeet, Singh Rajendra, Tiwari Ashok Kumar
Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, Bareilly, Uttar Pradesh, India.
Vet Ital. 2016 Sep 30;52(3-4):305-311. doi: 10.12834/VetIt.569.2715.2.
Bluetongue (BT) is a non‑contagious arthropod‑borne viral disease of domestic and wild ruminants. It is endemic to India and clinical outbreaks of disease have been reported mainly in sheep, although BT is often asymptomatic in other ruminant species. In the present serological survey, a total of 576 serum samples, comprising of 416 cattle and 160 sheep, covering different agro‑climatic zones of Rajasthan, Uttar Pradesh, and Karnataka states, were screened for the presence of Bluetongue virus (BTV) specific antibodies using competitive enzyme‑linked immunosorbent assay (c‑ELISA). Overall 73.08% (304/416) of the cattle and 53.30% (87/160) of the sheep serum samples were positive for BTV antibodies. The prevalence of BTV antibodies in cattle in different agro‑climatic zones ranged between 60‑80% in Rajasthan and 66‑70% in Uttar Pradesh. During the study, a nested polymerase chain reaction (PCR) based on the BTV NS1 gene (genome segment 5) was optimized for detection of BTV's nucleic acid from a cell adapted strain of BTV‑23, and field derived clinical blood samples. In the present study, 19/70 of cattle and 9/30 of sheep blood samples tested positive for BTV RNA by the nested PCR, which amplified specific products of 274 bp and 101 bp sizes, respectively. From this study, it can be concluded that cattle showed higher percentage of sero‑positivity in comparison to sheep. The improved sero‑surveillance system for BTV in endemic areas will be of great help to understand the epidemiology of BTV and to formulate effective control and preventive strategies.
蓝舌病（BT）是一种影响家养和野生反刍动物的非传染性节肢动物传播的病毒性疾病。该病在印度呈地方流行性，虽然蓝舌病在其他反刍动物物种中通常无症状，但主要在绵羊中报告有临床疾病暴发。在本次血清学调查中，使用竞争酶联免疫吸附测定（c-ELISA）对来自拉贾斯坦邦、北方邦和卡纳塔克邦不同农业气候区的总共576份血清样本进行了筛查，其中包括416份牛血清样本和160份绵羊血清样本，以检测蓝舌病病毒（BTV）特异性抗体。总体而言，73.08%（304/416）的牛血清样本和53.30%（87/160）的绵羊血清样本BTV抗体呈阳性。不同农业气候区牛的BTV抗体流行率在拉贾斯坦邦为60%-80%，在北方邦为66%-70%。在研究过程中，优化了基于BTV NS1基因（基因组片段5）的巢式聚合酶链反应（PCR），用于从BTV-23的细胞适应株和现场采集的临床血液样本中检测BTV的核酸。在本研究中，通过巢式PCR检测，19/70的牛血液样本和9/30的绵羊血液样本BTV RNA呈阳性，分别扩增出大小为274 bp和101 bp的特异性产物。从这项研究可以得出结论，与绵羊相比，牛的血清阳性率更高。在流行地区改进的BTV血清监测系统将有助于了解BTV的流行病学，并制定有效的控制和预防策略。
