Rojekittikhun W, Nuamtanong S, Pubampen S, Setasuban P
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1989 Mar;20(1):81-6.
Mice, rats and cats were infected either orally or percutaneously with a number of early or advanced third-stage larvae (EL3 or AL3, respectively) of G. spinigerum. Sera obtained from these infected animals and 10 human gnathostomiasis cases were tested against various developmental stages of the parasite which were prepared and used while being alive (fresh) or dead (air-dried) for the circumoval and larval microprecipitation (COP and LMP) reactions. No precipitin reactions were observed in all sera tested against unembryonated eggs, embryonated eggs and first stage larvae neither air-dried nor fresh preparations. Sera were merely reactive giving various degrees of membranous or filamentous precipitates against the air-dried preparation of AL3.
用多只棘颚口线虫的早期或晚期第三期幼虫（分别为EL3或AL3）经口或经皮感染小鼠、大鼠和猫。从这些受感染动物以及10例人类颚口线虫病病例中获取血清，针对寄生虫的不同发育阶段进行检测，这些发育阶段的寄生虫在存活（新鲜）或死亡（风干）状态下制备并用于环卵沉淀反应（COP）和幼虫微量沉淀反应（LMP）。在针对未受精卵、受精卵和第一期幼虫的所有血清检测中，无论是风干还是新鲜制剂，均未观察到沉淀素反应。血清仅对风干的AL3制剂产生反应，出现不同程度的膜状或丝状沉淀。
