Clements J R, Magnusson K R, Beitz A J
Department of Veterinary Biology, University of Minnesota, St. Paul 55108.
Synapse. 1989;4(1):70-9. doi: 10.1002/syn.890040108.
A monoclonal antibody against taurine conjugated to KLH was used to identify and describe taurine-like immunoreactive processes in the rat hippocampus. Tissue from perfused rats was processed for immunohistochemical visualization of taurine and embedded for electron microscopy. Representative tissue samples from three regions, the dentate gyrus, CA3, and CA1, were sectioned, examined, and photographed. In the dentate gyrus, both granule cells and pyramidal basket cells were taurine-like immunoreactive. Some axon terminals in the dentate gyrus molecular layer as well as some mossy fiber boutons in the hilus were also taurine-like immunoreactive. In the CA3 region both pyramidal neurons and glial cells were taurine-like immunoreactive A few small-diameter axon terminals in stratum radiatum and some mossy fiber boutons in stratum lucidum were taurine-like immunoreactive. In CA1, pyramidal neurons and some glia were intensely taurine-like immunoreactive. A few immunoreactive axon terminals were seen in stratum radiatum and stratum oriens. In all regions, dendritic staining predominated. Our results support the hypothesis that while taurine may act as a neurotransmitter in a small portion of hippocampal terminals, its main function is probably as a neuromodulator or ionic regulator.
一种与钥孔血蓝蛋白（KLH）偶联的抗牛磺酸单克隆抗体被用于识别和描述大鼠海马体中牛磺酸样免疫反应过程。对灌注大鼠的组织进行处理，以进行牛磺酸的免疫组织化学可视化，并包埋用于电子显微镜检查。对来自齿状回、CA3和CA1这三个区域的代表性组织样本进行切片、检查和拍照。在齿状回中，颗粒细胞和锥体细胞性篮状细胞均呈牛磺酸样免疫反应。齿状回分子层中的一些轴突终末以及门区中的一些苔藓纤维终扣也呈牛磺酸样免疫反应。在CA3区域，锥体细胞和胶质细胞均呈牛磺酸样免疫反应。辐射层中的一些小直径轴突终末和透明层中的一些苔藓纤维终扣呈牛磺酸样免疫反应。在CA1中，锥体细胞和一些胶质细胞呈强烈的牛磺酸样免疫反应。在辐射层和原层中可见一些免疫反应性轴突终末。在所有区域中，树突染色占主导。我们的结果支持这样一种假说，即虽然牛磺酸可能在一小部分海马体终末中作为神经递质起作用，但其主要功能可能是作为神经调节剂或离子调节剂。
