Techna Institute for the Advancement of Technology for Health, University Health Network, Toronto, ON, M5G-1P5, Canada.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.
J Am Soc Mass Spectrom. 2017 Jan;28(1):145-153. doi: 10.1007/s13361-016-1506-x. Epub 2016 Oct 11.
Mass spectrometry imaging with desorption electrospray ionization mass spectrometry (DESI-MS) is used to characterize cancer from ex vivo slices of tissues. The process is time-consuming. The use of tissue smears for DESI-MS analysis has been proposed as it eliminates the time required to snap-freeze and section the tissue. To assess the utility of tissue smears for rapid cancer characterization, principal component analysis (PCA) was performed to evaluate the concordance between DESI-MS profiles of breast cancer from tissue slices and smears prepared on various surfaces. PCA suggested no statistical discrimination between DESI-MS profiles of tissue sections and tissue smears prepared on glass, polytetrafluoroethylene (PTFE), and porous PTFE. However, the abundances of cancer biomarker ions varied between sections and smears, with DESI-MS analysis of tissue sections yielding higher ion abundances of cancer biomarkers compared with smears. Coefficient of variance (CV) analysis suggests DESI-MS profiles from tissue smears are as reproducible as the ones from tissue sections. The limit of detection with smear samples from single pixel analysis is comparable to tissue sections that average the signal from a tissue area of 0.01 mm. The smears prepared on the PTFE surface possessed a higher degree of homogeneity compared with the smears prepared on the glass surface. This allowed single MS scans (~1 s) from random positions across the surface of the smear to be used in rapid cancer typing with good reproducibility, providing pathologic information for cancer typing at speeds suitable for clinical utility. Graphical Abstract ᅟ.
利用解吸电喷雾电离质谱（DESI-MS）进行质谱成像，可对组织的离体切片进行癌症特征分析。该过程耗时较长。已经提出了使用组织涂片进行 DESI-MS 分析，因为它可以消除对组织进行快速冷冻和切片所需的时间。为了评估组织涂片在快速癌症特征分析中的效用，进行了主成分分析（PCA），以评估乳腺癌的 DESI-MS 谱在组织切片和在各种表面上制备的涂片之间的一致性。PCA 表明，组织切片和在玻璃、聚四氟乙烯（PTFE）和多孔 PTFE 上制备的涂片的 DESI-MS 谱之间没有统计学上的区别。然而，组织切片和涂片之间的癌症生物标志物离子丰度存在差异，与涂片相比，组织切片的 DESI-MS 分析产生了更高的癌症生物标志物离子丰度。变异系数（CV）分析表明，组织涂片的 DESI-MS 谱与组织切片的谱一样具有可重复性。从单个像素分析的涂片样本中检测到的限，与从组织面积为 0.01mm 的组织区域平均信号的组织切片相当。与在玻璃表面上制备的涂片相比，在 PTFE 表面上制备的涂片具有更高的均匀性。这允许从涂片表面的随机位置进行单次 MS 扫描（约 1 秒），以进行快速癌症分型，具有良好的可重复性，为癌症分型提供了病理信息，速度适合临床应用。
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