Department of Chemistry, The University of Texas at Austin, Austin, TX, 78712, USA.
Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, 77030, USA.
J Am Soc Mass Spectrom. 2017 Jun;28(6):1166-1174. doi: 10.1007/s13361-016-1570-2. Epub 2017 Feb 28.
Ambient ionization mass spectrometry has been widely applied to image lipids and metabolites in primary cancer tissues with the purpose of detecting and understanding metabolic changes associated with cancer development and progression. Here, we report the use of desorption electrospray ionization mass spectrometry (DESI-MS) to image metastatic breast and thyroid cancer in human lymph node tissues. Our results show clear alterations in lipid and metabolite distributions detected in the mass spectra profiles from 42 samples of metastatic thyroid tumors, metastatic breast tumors, and normal lymph node tissues. 2D DESI-MS ion images of selected molecular species allowed discrimination and visualization of specific histologic features within tissue sections, including regions of metastatic cancer, adjacent normal lymph node, and fibrosis or adipose tissues, which strongly correlated with pathologic findings. In thyroid cancer metastasis, increased relative abundances of ceramides and glycerophosphoinisitols were observed. In breast cancer metastasis, increased relative abundances of various fatty acids and specific glycerophospholipids were seen. Trends in the alterations in fatty acyl chain composition of lipid species were also observed through detailed mass spectra evaluation and chemical identification of molecular species. The results obtained demonstrate DESI-MSI as a potential clinical tool for the detection of breast and thyroid cancer metastasis in lymph nodes, although further validation is needed. Graphical Abstract Desorption electrospray ionization mass spectrometry imaging is used to differentiate metastatic cancer from adjacent lymph node tissue.
环境解析电离质谱已广泛应用于对原发性癌症组织中的脂质和代谢物进行成像，目的是检测和了解与癌症发展和进展相关的代谢变化。在这里，我们报告了使用解吸电喷雾电离质谱（DESI-MS）对人淋巴结组织中的转移性乳腺癌和甲状腺癌进行成像。我们的结果表明，在 42 个转移性甲状腺肿瘤、转移性乳腺癌和正常淋巴结组织的质谱图谱中，检测到脂质和代谢物分布的明显改变。选定分子种类的 2D DESI-MS 离子图像允许对组织切片中的特定组织学特征进行区分和可视化，包括转移性癌症、相邻正常淋巴结、纤维化或脂肪组织的区域，这与病理发现有很强的相关性。在甲状腺癌转移中，观察到神经酰胺和甘油磷酸肌醇的相对丰度增加。在乳腺癌转移中，观察到各种脂肪酸和特定甘油磷脂的相对丰度增加。通过详细的质谱评估和分子种类的化学鉴定，还观察到脂质种类中脂肪酸链组成变化的趋势。所得结果表明，DESI-MSI 可作为一种潜在的临床工具，用于检测淋巴结中的乳腺癌和甲状腺癌转移，但需要进一步验证。 图表摘要 解吸电喷雾电离质谱成像用于区分转移性癌症与相邻的淋巴结组织。
