细胞核与核仁的分离

Isolation of Nuclei and Nucleoli.

作者信息

Pendle Alison F, Shaw Peter J

机构信息

Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.

出版信息

Methods Mol Biol. 2017;1511:31-44. doi: 10.1007/978-1-4939-6533-5_3.

DOI:10.1007/978-1-4939-6533-5_3

PMID:27730600

Abstract

Here we describe methods for producing nuclei from Arabidopsis suspension cultures or root tips of Arabidopsis, wheat, or pea. These methods could be adapted for other species and cell types. The resulting nuclei can be further purified for use in biochemical or proteomic studies, or can be used for microscopy. We also describe how the nuclei can be used to obtain a preparation of nucleoli.

摘要

在此，我们描述了从拟南芥悬浮培养物或拟南芥、小麦或豌豆的根尖中制备细胞核的方法。这些方法可适用于其他物种和细胞类型。所得到的细胞核可进一步纯化，用于生化或蛋白质组学研究，或用于显微镜观察。我们还描述了如何利用这些细胞核来获得核仁制剂。