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[非酶法分离并体外培养的大鼠肝细胞的功能特性]

[Functional characteristics of rat hepatocytes isolated by a nonenzymatic method and incubated in vitro].

作者信息

Petrenko A Iu, Andrienko A N, Kravchenko L P, Sukach A N

出版信息

Tsitologiia. 1989 May;31(5):608-11.

PMID:2773068
Abstract

A study was made of the effect of normothermic incubation on the metabolic state of parenchymal liver cells, isolated by the nonenzymatic method. In the process of a 2 hour incubation in the nutritious sodium-containing medium, a high level of the energy supply system is maintained, determined by the contents of adenine nucleotides, the rate of endogenous respiration and by its stimulation by succinate.

摘要

对采用非酶法分离的肝实质细胞在常温孵育条件下其代谢状态的影响进行了一项研究。在含钠营养培养基中孵育2小时的过程中,通过腺嘌呤核苷酸含量、内源性呼吸速率及其受琥珀酸刺激的情况来确定,能量供应系统维持在较高水平。

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