Mohamad Isa Irma Izani, Abu Bakar Suhaili, Md Tohid Siti Farah, Mat Jais Abdul Manan
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Ethnopharmacol. 2016 Dec 24;194:469-474. doi: 10.1016/j.jep.2016.10.033. Epub 2016 Oct 11.
Haruan, Channa striatus, is a freshwater fish which has been well-known locally to accelerate wound healing during post-operative and post-partum periods. The fish extract also has potent anti-inflammatory and analgesic properties.
To assess topical anti-inflammatory effect of Haruan cream on 12-0-tetradecanoylphorbol-13-acetate (TPA)-induced chronic-like dermatitis in mice.
Male ICR mice were randomized into six groups of five mice each: acetone (vehicle), TPA alone (negative control), three Haruan treatment groups (Haruan 1%, Haruan 5% and Haruan 10%) and hydrocortisone 1% (positive control). Briefly, both surfaces of mouse ears were applied with TPA (2.5μg/20μl acetone) for five times on alternate days and with Haruan or hydrocortisone 1% cream for the last three days. Mouse ear thickness was measured 24h after final treatment with the cream and the ears were harvested for further histological analysis and gene expression studies of TNF-α by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR).
Topical application of Haruan cream had reduced the mouse ear thickness 18.1-28%) with comparable effect to the positive control. In addition, histopathological comparison had shown evident reduction in various parameters of cutaneous inflammation including dermal oedema, inflammatory cells infiltration and proliferation of epidermal keratinocytes. Furthermore, TPA application had resulted in the up-regulation of TNF-α gene expression by 353-fold, which was subsequently down-regulated by the Haruan cream (34- to 112-fold).
Haruan is an effective topical anti-inflammatory agent in this mouse model of chronic-like dermatitis, thus suggesting its potential as a non-steroidal treatment option for chronic inflammatory dermatoses.
哈鲁安鱼，即条纹鲶鱼，是一种淡水鱼，在当地广为人知的是它能加速术后和产后伤口愈合。这种鱼提取物还具有强大的抗炎和镇痛特性。
评估哈鲁安乳膏对12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）诱导的小鼠慢性皮炎样炎症的局部抗炎作用。
将雄性ICR小鼠随机分为六组，每组五只：丙酮组（赋形剂）、单独TPA组（阴性对照）、三个哈鲁安治疗组（1%哈鲁安、5%哈鲁安和10%哈鲁安）和1%氢化可的松组（阳性对照）。简要地说，在小鼠双耳表面隔天涂抹TPA（2.5μg/20μl丙酮）共五次，在最后三天涂抹哈鲁安或1%氢化可的松乳膏。在最后一次涂抹乳膏24小时后测量小鼠耳厚度，并取下耳朵进行进一步的组织学分析以及通过实时逆转录聚合酶链反应（RT - qPCR）对肿瘤坏死因子 - α（TNF - α）进行基因表达研究。
局部应用哈鲁安乳膏可使小鼠耳厚度降低18.1% - 28%，效果与阳性对照相当。此外，组织病理学比较显示皮肤炎症的各种参数明显降低，包括真皮水肿、炎性细胞浸润和表皮角质形成细胞增殖。此外，TPA的应用导致TNF - α基因表达上调353倍，随后哈鲁安乳膏使其下调（34至112倍）。
在这种慢性皮炎样小鼠模型中，哈鲁安是一种有效的局部抗炎剂，因此表明其作为慢性炎症性皮肤病非甾体治疗选择的潜力。
