a State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China.
b Yanbian University Hospital , Yanji , China , and.
J Liposome Res. 2018 Mar;28(1):5-13. doi: 10.1080/08982104.2016.1239633. Epub 2016 Nov 23.
Keloids were characterized by excessive growth of fibrous tissues, and shared several pathological characteristics with cancer. They did put physical and emotional stress on patients in that keloids could badly change appearance of patients. N-(4-hydroxyphenyl) retinamide (4HPR) showed cytotoxic activity on a wide variety of invasive-growth cells. Our work was aim to prepare N-(4-hydroxyphenyl) retinamide-loaded lipid microbubbles (4HPR-LM) combined with ultrasound for anti-keloid therapy. 4HPR-loaded liposomes (4HPR-L) were first prepared by film evaporation method, and then 4HPR-LM were manufactured by mixing 4HPR-L and perfluoropentane (PFP) with ultrasonic cavitation method. The mean particle size and entrapment efficiency 4HPR-LM were 113 nm and 95%, respectively. The anti-keloids activity of 4HPR-LM was assessed with BALB/c nude mice bearing subcutaneous xenograft keloids model. 4HPR-LM, combined with ultrasound, could significantly induce apoptosis of keloid fibroblasts in vitro and inhibited growth of keloids in vivo. Thus, 4HPR-LM could be considered as a promising agent for anti-keloids therapy.
瘢痕疙瘩的特征是纤维组织过度生长，并具有与癌症相似的一些病理特征。它们确实给患者带来了身体和情感上的压力，因为瘢痕疙瘩会严重改变患者的外貌。N-(4-羟基苯基)维甲酸（4HPR）对多种侵袭性生长细胞表现出细胞毒性作用。我们的工作旨在制备载 N-(4-羟基苯基)维甲酸的脂质微泡（4HPR-LM）并结合超声用于抗瘢痕疙瘩治疗。首先采用薄膜蒸发法制备载 4HPR 的脂质体（4HPR-L），然后通过超声空化法将 4HPR-L 和全氟戊烷（PFP）混合制备 4HPR-LM。4HPR-LM 的平均粒径和包封效率分别为 113nm 和 95%。采用 BALB/c 裸鼠皮下移植瘢痕疙瘩模型评估 4HPR-LM 的抗瘢痕疙瘩活性。4HPR-LM 联合超声可显著诱导体外瘢痕疙瘩成纤维细胞凋亡，并抑制体内瘢痕疙瘩生长。因此，4HPR-LM 可被认为是一种有前途的抗瘢痕疙瘩治疗药物。
