Xu Jie, Yan Rui
Department of Foreign Languages, Chaohu University, Chaohu 238000, P.R. China.
College of Pharmacy, Liaoning University, 66 Chongshan Road (M), Shenyang 110036, P.R. China
J Chromatogr Sci. 2017 Feb;55(2):162-165. doi: 10.1093/chromsci/bmw165. Epub 2016 Oct 12.
This study aims to study the changes of metabolism of Ephedra sinica Stapf caused by processing. A sensitive and rapid high-performance liquid chromatographic tandem mass spectrometry (LC-MS-MS) method was developed for the quantitation of the major metabolite of ephedrine, 4-hydroxyephedrine, utilizing lidocaine as the internal standard in rat urine. Urine samples were precipitated with acetonitrile. Chromatographic separation was achieved on an Ultimate C18 analytical column. Detection was performed by a multiple reaction monitoring mode via an electrospray ionization source operating in the positive ionization mode. The method was linear over the concentration range of 0.05-1.0c for all components. The intra- and inter-day precision values were <13.2% and the deviations ranged from -8.4% to 7.5%. The recoveries at three levels were more than 66.2%. The fully validated method was used to study the pharmacokinetic profile of 4-hydroxyephedrine in rat urine to investigate the effects caused by processing.
本研究旨在探讨炮制对麻黄代谢的影响。建立了一种灵敏、快速的高效液相色谱串联质谱（LC-MS-MS）法，以利多卡因为内标，定量大鼠尿液中麻黄碱的主要代谢产物4-羟基麻黄碱。尿液样品用乙腈沉淀。采用Ultimate C18分析柱进行色谱分离。通过电喷雾电离源在正离子模式下运行的多反应监测模式进行检测。该方法对所有组分在0.05-1.0c浓度范围内呈线性。日内和日间精密度值均<13.2%，偏差范围为-8.4%至7.5%。三个水平的回收率均超过66.2%。该经过充分验证的方法用于研究大鼠尿液中4-羟基麻黄碱的药代动力学特征，以探讨炮制的影响。
