在大肠杆菌中生产重组CCN2蛋白。

Production of Recombinant CCN2 Protein in Escherichia coli.

作者信息

Aoyama Eriko, Hattori Takako, Kubota Satoshi, Takigawa Masaharu

机构信息

Advanced Research Center for Oraland Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.

Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.

出版信息

Methods Mol Biol. 2017;1489:77-84. doi: 10.1007/978-1-4939-6430-7_8.

DOI:10.1007/978-1-4939-6430-7_8

PMID:27734367

Abstract

Recombinant proteins are important tools for understanding molecular functions in vitro. Recent progress in the generation of recombinant proteins is amazing. However, when we plan to produce them, we should choose the best method according to the nature and the use of the target recombinant protein. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in Escherichia coli.

摘要

重组蛋白是体外理解分子功能的重要工具。重组蛋白生成方面的最新进展令人惊叹。然而，当我们计划生产重组蛋白时，应根据目标重组蛋白的性质和用途选择最佳方法。降解和错误折叠是生产活性重组CCN2时的主要问题。本章所示方法描述了在大肠杆菌中生产CCN2及其截短片段的合适条件。