Kubota Satoshi, Kawaki Harumi, Takigawa Masaharu
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
Department of MembraneBiochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Methods Mol Biol. 2017;1489:127-138. doi: 10.1007/978-1-4939-6430-7_13.
Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.
酶联免疫吸附测定（ELISA）是用于绝对定量液体样品中特定蛋白质的最常用方法。特别是对于与人类疾病相关的CCN家族成员，强调了ELISA在临床以及研究实验室中对这些蛋白质的实用性。然而，为了在ELISA中获得准确和稳定的结果，在控制标准CCN家族蛋白质的质量和数量时应格外小心，这些蛋白质会与各种材料结合，并且以纯化形式可能不稳定。最近，CCN家族蛋白质片段在几种疾病的体液中的诊断价值已得到证实。因此，作为一种有前景的临床工具，需要针对CCN家族成员的模块特异性检测系统。还应注意的是，CCN家族成员的模块化片段在纯化形式下可能比全长片段更稳定，其质量可能比全长片段更容易控制。
